West Nile Virus Vaccination Protects against Usutu Virus Disease in Mice
| Autor: | Ana Vázquez, Claire Y.-H. Huang, Rebecca M Salgado, Francesca Frere, Seth A. Hawks, Nisha K. Duggal |
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| Přispěvatelé: | NIH - National Institute of Allergy and Infectious Diseases (NIAID) (Estados Unidos), Virginia–Maryland College of Veterinary Medicine (Estados Unidos) |
| Rok vydání: | 2021 |
| Předmět: |
Male
Neutralizing response animal diseases viruses neutralizing response Viremia Dengue virus Antibodies Viral medicine.disease_cause Microbiology Article Flavivirus Infections Mice Antigen vaccine Virology medicine Animals West Nile Virus Vaccines Usutu virus Mice Knockout biology Flavivirus Vaccination virus diseases biology.organism_classification medicine.disease Antibodies Neutralizing QR1-502 nervous system diseases Titer Infectious Diseases biology.protein Female flavivirus West Nile virus Antibody Vaccine |
| Zdroj: | Repisalud Instituto de Salud Carlos III (ISCIII) Viruses; Volume 13; Issue 12; Pages: 2352 Viruses Viruses, Vol 13, Iss 2352, p 2352 (2021) |
| Popis: | West Nile virus (WNV) and Usutu virus (USUV) are mosquito-borne flaviviruses that can cause neuroinvasive disease in humans. WNV and USUV circulate in both Africa and Europe and are closely related. Due to antigenic similarity, WNV-specific antibodies and USUV-specific antibodies have the potential to bind heterologous viruses; however, it is unclear whether this interaction may offer protection against infection. To investigate how prior WNV exposure would influence USUV infection, we used an attenuated WNV vaccine that contains the surface proteins of WNV in the backbone of a dengue virus 2 vaccine strain and protects against WNV disease. We hypothesized that vaccination with this attenuated WNV vaccine would protect against USUV infection. Neutralizing responses against WNV and USUV were measured in vitro using sera following vaccination. Sera from vaccinated CD-1 and Ifnar1-/- mice cross-neutralized with WNV and USUV. All mice were then subsequently challenged with an African or European USUV strain. In CD-1 mice, there was no difference in USUV titers between vaccinated and mock-vaccinated mice. However, in the Ifnar1-/- model, vaccinated mice had significantly higher survival rates and significantly lower USUV viremia compared to mock-vaccinated mice. Our results indicate that exposure to an attenuated form of WNV protects against severe USUV disease in mice and elicits a neutralizing response to both WNV and USUV. Future studies will investigate the immune mechanisms responsible for the protection against USUV infection induced by WNV vaccination, providing critical insight that will be essential for USUV and WNV vaccine development. Funding for this project was provided by NIH NIAID R21 AI53988. Support was also provided by the Virginia–Maryland College of Veterinary Medicine IRC. Sí |
| Databáze: | OpenAIRE |
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