Analytical method for sequential determination of persistent herbicides and their metabolites in fish tissues by UPLC-MS/MS
| Autor: | Xuesheng Li, Sheng Liu, Dongqiang Zeng, Cuifang Zhang, Zhuang Wang, Huihua Tan |
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| Rok vydání: | 2021 |
| Předmět: |
Detection limit
Analyte China Environmental Engineering Chromatography Chemistry Herbicides Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health General Medicine General Chemistry Mass spectrometry Quechers Pollution chemistry.chemical_compound Tandem Mass Spectrometry Bioaccumulation Environmental Chemistry Animals Acetochlor Atrazine Metolachlor Chromatography High Pressure Liquid Chromatography Liquid |
| Zdroj: | Chemosphere. 288(Pt 2) |
| ISSN: | 1879-1298 |
| Popis: | A novel and accurate liquid chromatography–tandem mass spectrometry method was developed to sequentially determine three persistent herbicides (atrazine (ATZ), acetochlor (ACE), and metolachlor (MET)) and seven characteristic metabolites (desethylatrazine (DEA), deisopropylatrazine (DIA), diaminochlorotriazine (DACT), MET–oxanilic acid (MET-OA), MET-ethanesulfonic acid (MET-ESA), ACE-ESA, and ACE-OA) in fresh fish tissues from six fish species. A modified QuEChERS method was conducted to extract the target compounds from fish tissues. Matrix-matched calibrations of the target analytes were carried out at spiking levels of 1, 10, 100, and 1000 ng g−1. The method was validated in accordance with Codex guidelines (CAC/GL 71–2009). Recoveries for the target analytes were 67–120% with relative standard deviations below 20%, and the matrix effects ranged from −58.7% to 59.3%. The limits of detection and quantitation were 0.01–1.90 and 0.02–6.35 ng g−1, respectively. Moreover, the method was successfully applied to analyze the concentrations of the target chemicals in fresh tissue samples of six fish species (n = 67) collected from four markets in Nanning City, Guangxi Province, China. The concentrations in all samples were 1.1–140.5 ng g−1. Interestingly, this study was the first to measure DEA and DIA in fish liver, and their highest concentrations were 10.7 and 14.2 ng g−1, respectively. This method provides a basis for studying the pathways of biotransformation, bioaccumulation, detoxification, and exposure patterns of ACE, ATZ, MET, and their metabolites in aquatic environments. |
| Databáze: | OpenAIRE |
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