| Popis: |
Immune thrombocytopenia (ITP) is an autoimmune hemorrhagic disease with distinct clinical manifestations such as extensive skin petechiae, mucosal bleeding, and even visceral hemorrhage. In this study, CD3+T lymphocytes from ITP patients were screened for differentially expressed genes. The expression of miR-21 and miR-155 in T lymphocytes of ITP patients were investigated. The downstream target genes of miR-21 and miR-155 were also searched for the correlation between differentially expressed genes of ITP.Differential gene screening was performed using the GSE43177 data set in the GEO database, and the expression of miR-21 and miR-155 in T lymphocytes of ITP patients was verified by qPCR. The interaction network of core downstream target genes and ITP differentially expressed genes of miR-21 and miR-155 were constructed with the STRING database, and the associated factors were verified by qPCR.In ITP patients, the expression of CD8+T lymphocytes increased, the expression of CD4+T lymphocytes decreased, and the ratio of CD4+/CD8+T cells decreased. Fourteen genes were differentially expressed in CD3+T lymphocytes, all of which were upregulated, and the expression of S100A8 was increased in ITP patients. The expression of miR-21-5p and miR-155-5p increased in CD3+T lymphocytes of initial ITP patients. The core down-stream target gene VCL of miR-21 was associated with the differentially expressed genes such as LTF, LCN2, and DEFA4 in the interaction network. VCL expression was decreased and LTF expression was increased in ITP patients.S100A8 plays an important role in the regulation of CD3+T lymphocytes in ITP patients. MiR-21-5p regulates the differentially expressed gene LTF by inhibiting the core downstream target gene VCL and participates in the immune mechanism of T lymphocytes in ITP patients. MiR-155-5p is also involved in the immunoregulatory mechanism of T lymphocytes in ITP patients. |
