Tremella fuciformis enhances the neurite outgrowth of PC12 cells and restores trimethyltin-induced impairment of memory in rats via activation of CREB transcription and cholinergic systems
| Autor: | Insop Shim, Yong Ho Ahn, Ik Hyun Yeo, Jung Il Kang, Hyun Soo Shim, Hyo-Cheol Ha, Woong Ki Choi, Kum Ju Park, Jin Su Kim, Kyung Soo Kim, Hyun Jung Park, Tae Seok Kim |
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| Rok vydání: | 2011 |
| Předmět: |
CAMP Responsive Element Binding Protein
Male medicine.medical_specialty Time Factors Neurite Cholinergic Agents Morris water navigation task Hippocampal formation CREB Hippocampus PC12 Cells Choline O-Acetyltransferase Rats Sprague-Dawley Behavioral Neuroscience Fluorodeoxyglucose F18 Polysaccharides Internal medicine medicine Neurites Animals Maze Learning Analysis of Variance Memory Disorders biology Dose-Response Relationship Drug Trimethyltin Compounds Chemistry Choline acetyltransferase CREB-Binding Protein Rats Endocrinology Neuroprotective Agents Frontal lobe Gene Expression Regulation Positron-Emission Tomography biology.protein Cholinergic Neuroscience |
| Zdroj: | Behavioural brain research. 229(1) |
| ISSN: | 1872-7549 |
| Popis: | The present study examined the effects of Tremella fuciformis (TF) on the learning and memory function and the neural activity in rats with trimethyltin (TMT)-induced memory deficits. The rats were administered saline or TF (TF 25, 50, 100 mg/kg, p.o.) daily for 21 days. The cognitive improving efficacy of TF on the amnesic rats, which was induced by TMT, was investigated by assessing the Morris water maze test and by performing Choline acetyltransferase (ChAT) and cAMP responsive element binding protein (CREB) immunohistochemistry. In order to confirm the underlying mechanisms of the memory enhancing effects of TF, we assessed the neurite outgrowth of PC12 cells. We also administered 18F-fluorodeoxyglucose and performed a PET scan of the frontal lobe. The rats with TMT injection showed impaired learning and memory of the tasks and treatment with TF produced a significant improvement of the escape latency to find the platform in the Morris water maze compared to that of the control group. In the retention test, the TF50 group showed increased time spent around the platform compared to that of the control group. Consistent with the behavioral data, TF50 mg/kg significantly alleviated the loss of ChAT-ir neurons in the hippocampus compared to that of the control group. Treatment with TF significantly increased the CREB positive neurons in the hippocampal CA1 area as compared to that of the control group. In addition, TF treatment (50 mg/kg) increased the glucose uptake approximately sevenfold in the frontal lobe and it significantly promoted neurite outgrowth of the PC12 cells, as compared to that of the controls. These results suggest that TF may be useful for improving the cognitive function via regulation of the CREB signaling pathway and cholinergic system in the hippocampus. |
| Databáze: | OpenAIRE |
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