The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue
| Autor: | Andrea Dlasková, Kieran Clarke, Richard K. Porter |
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| Jazyk: | angličtina |
| Předmět: |
Male
Biophysics chemistry.chemical_element Adipose tissue Mitochondrion Biology Brown adipose tissue Biochemistry Oxygen Ion Channels Mitochondrial Proteins 03 medical and health sciences Mice 0302 clinical medicine Oxygen Consumption Adipose Tissue Brown medicine Animals 030304 developmental biology chemistry.chemical_classification Membrane potential 0303 health sciences Reactive oxygen species Uncoupling protein 1 Cell Biology Electron transport chain Thermogenin Mitochondria Mice Inbred C57BL medicine.anatomical_structure chemistry Female 030217 neurology & neurosurgery |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. (8):1470-1476 |
| ISSN: | 0005-2728 |
| DOI: | 10.1016/j.bbabio.2010.04.008 |
| Popis: | We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wild-type mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. |
| Databáze: | OpenAIRE |
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