Opposite cellular accumulation and nitric oxide production in vivo after pleural immunization with M. leprae or M. bovis BCG
| Autor: | E C Rosas, R. S. B. Cordeiro, M G Henriques, E Werneck-Barroso, A. C. N. Moura |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Biology Nitric Oxide Nitric oxide Mice chemistry.chemical_compound Antigen Cell Movement In vivo Genetics medicine Animals Eosinophilia Macrophage Pleurisy Mice Inbred BALB C Dose-Response Relationship Drug Vaccination General Medicine Pleural cavity medicine.disease Mycobacterium bovis Mycobacterium leprae Kinetics medicine.anatomical_structure chemistry Bacterial Vaccines Immunology Pleura medicine.symptom Epithelioid cell |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.3.1.69 |
| Popis: | Mycobacteria as intracellular pathogens have evolved mechanisms to survive within macrophages. Our previous data showed that M. leprae (ML), unlike M. bovis BCG, did not induce an inflammatory response in the mice subcutaneous tissue. Further, ML inhibited BCG-induced foot pad oedema and seemed to transform macrophages in epithelioid cells. Since these mycobacteria share common antigens, here we seeked to compare the acute and chronic cellular response evoked by ML and BCG in pleurisy of a mycobacteria-susceptible mice (BALB/c). The total leukocytes, the cell type that migrated to the pleural cavity and macrophage activation assayed by nitric oxide release were determined. Live or dead BCG Moreau recruited the same extent of cells, essentially monocytes and neutrophils, dose-dependently, in both acute and chronic pleurisy. BCG-induced eosinophilia was observed only in the acute response (after 24 h of injection). A significant nitric oxide release by pleural macrophages was triggered by BCG Moreau without previous activation. Nevertheless, ML failed to recruit leukocytes to the pleural space or to lead to nitric oxide production despite the number of bacilli used and the time studied (1, 7 or 14 days after injection). Although these mycobacteria have common antigens that cross-react, these data show a distinct ability of ML or BCG to recruit cells to the pleural space and to activate pleural macrophage for nitric oxide production in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|