Weaning differentially affects mitochondrial function, oxidative stress, inflammation and apoptosis in normal and low birth weight piglets

Autor: C. Roy, J. Jacques Matte, Yan Martel-Kennes, Martin Lessard, Karine Deschene, Aliny K. Novais, Jean-Paul Laforest, Jérôme Lapointe
Rok vydání: 2021
Předmět:
GPX3
Swine
Physiology
animal diseases
Interleukin-1beta
Gene Expression
Apoptosis
Mitochondrion
medicine.disease_cause
Biochemistry
Intestinal mucosa
Immune Physiology
Medicine and Health Sciences
Birth Weight
Intestinal Mucosa
Energy-Producing Organelles
Mammals
Innate Immune System
Multidisciplinary
Eukaryota
Mitochondria
Up-Regulation
Mitochondrial respiratory chain
Physiological Parameters
Vertebrates
Cytokines
Medicine
Cellular Structures and Organelles
Anatomy
medicine.symptom
Research Article
Science
Immunology
Down-Regulation
Inflammation
Weaning
Bioenergetics
Biology
Andrology
Proto-Oncogene Proteins
Genetics
medicine
Animals
Body Weight
Organisms
Biology and Life Sciences
Cell Biology
Molecular Development
Gastrointestinal Tract
Chemokine CXCL10
Oxidative Stress
Metabolism
Immune System
Amniotes
Chemokine CCL19
Energy Metabolism
Physiological Processes
Zoology
Digestive System
Heme Oxygenase-1
Oxidative stress
Developmental Biology
Zdroj: PLoS ONE, Vol 16, Iss 2, p e0247188 (2021)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0247188
Popis: Weaning is associated with increased occurrence of infections and diseases in piglets. Recent findings indicate that weaning induces mitochondrial dysfunction and oxidative stress conditions that more severely impact smaller piglets. The objective of this study was to characterize the molecular mechanisms underlying these physiological consequences and the relation with systemic inflammatory status in both normal and low birth weight (NBW and LBW) piglets throughout the peri-weaning period. To conduct the study, 30 sows were inseminated, and specific piglets from their litters were assigned to one of two experimental groups: NBW (n = 60, 1.73 ± 0.01 kg,) and LBW piglets weighing less than 1.2 kg (n = 60, 1.01 ± 0.01 kg). Then, 10 piglets from each group were selected at 14, 21 (weaning), 23, 25, 29 and 35 days of age to collect organ and plasma samples. Specific porcine RT2 Profiler™ PCR Arrays related to mitochondrial function, oxidative stress, inflammation and apoptosis processes were first used to target genes that are modulated after weaning in NBW piglets (d 23 and d 35 vs. d 14). Expression of selected genes was evaluated by quantitative PCR. These analyses revealed that expression of inflammatory genes CXCL10 and CCL19 increased after weaning in intestinal mucosa, while expression of genes encoding subunits of the mitochondrial respiratory chain was downregulated in liver and kidney of both groups. Interestingly, major modulators of mitophagy (BNIP3), cell survival (BCL2A1) and antioxidant defense system (TXNRD2, GPx3, HMOX1) were found to be highly expressed in NBW piglets. The systemic levels of TNF-α and IL1-β significantly increased following weaning and were higher in NBW piglets. These results provide novel information about the molecular origin of mitochondrial dysfunction and oxidative stress observed in weaned piglets and suggest that clearance of dysfunctional mitochondria, antioxidant defenses and inflammatory response are compromised in LBW piglets.
Databáze: OpenAIRE
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