Comparative evaluation of cell- and serum-derived exosomes to deliver immune stimulators to lymph nodes
| Autor: | Yoon Young Kang, Gyeonghui Yu, Heesun Jung, Hyejung Mok |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CD3 Complex CpG Oligodeoxynucleotide T-Lymphocytes T cell Biophysics Monophosphoryl Lipid A Bioengineering Exosomes Exosome Biomaterials Mice 03 medical and health sciences Immune system medicine Animals Antigen-presenting cell Lymph node Cells Cultured Chemistry Macrophages Microvesicles Cell biology Mice Inbred C57BL Lipid A RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure Oligodeoxyribonucleotides Mechanics of Materials NIH 3T3 Cells Ceramics and Composites Cattle Female Lymph Nodes |
| Zdroj: | Biomaterials. 162:71-81 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2018.02.003 |
| Popis: | To determine whether exosomes are efficient carriers for immune stimulating molecules into lymph nodes, comparative studies of exosomes (EXOs) derived from different origins (cells and serums) in terms of physicochemical properties and delivery efficiency were performed. Serum-derived EXOs were of a preferable size and generated higher yields than RAW264.7 cell-derived exosomes (RAW-EXO). In particular, fetal bovine serum-derived exosomes (bo-EXO), with a size below 50 nm, were delivered not only to surface zones (subcapsular sinus (SCS) macrophage zone) but also to inner paracortex zones (T cell zone) of lymph nodes, which allowed an efficient delivery of immune stimulating molecules to antigen presenting cells and T cells. The encapsulation of immune stimulating biomolecules (monophosphoryl lipid A (MPLA) and CpG oligodeoxynucleotides (CpG ODN)) within EXOs greatly increased intracellular delivery to macrophages via phagocytic pathways, which induced higher TNF-α and IL-6 secretion than free MPLA and free CpG ODN. MPLA-incorporated exosomes activated and differentiated T cells after subcutaneous injection, which elevated cytokine IFN-γ and TNF-α induction for CD3+ T cells. Taken together, bo-EXOs might serve as efficient carrier systems of immune stimulators to lymph nodes for desired immune responses. |
| Databáze: | OpenAIRE |
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