Suppression of Leaf Feeding and Oviposition of Phytophagous Ladybird Beetles (Coleoptera: Coccinellidae) by Chitinase Gene-transformed Phylloplane Bacteria and Their Specific Bacteriophages Entrapped in Alginate Gel Beads
| Autor: | Kenji Komuta, Teruo Nonomura, Yosiiinori Matsuda, Shigeyuki Mayama, Yasuyuki Sakuratani, Yukio Tosa, Souta Nogawa, Mori Hirofumi, Otsu Yasunari, Hideyoshi Toyoda, Hiroyuki Shimizu |
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| Rok vydání: | 2003 |
| Předmět: |
Lysis
Alginates Oviposition media_common.quotation_subject Insect medicine.disease_cause Insect Control Microbiology Solanum lycopersicum Enterobacter cloacae medicine Animals Bacteriophages Escherichia coli media_common Ecology biology Chitinases Feeding Behavior General Medicine biology.organism_classification Microspheres Coleoptera Plant Leaves Transformation (genetics) Insect Science Chitinase biology.protein Coccinellidae Transformation Bacterial Phyllosphere Gels Bacteria |
| Zdroj: | Journal of Economic Entomology. 96:555-563 |
| ISSN: | 1938-291X 0022-0493 |
| DOI: | 10.1093/jee/96.3.555 |
| Popis: | The chitinase gene-transformed strain KPM-007E/chi of Enterobacter cloacae was vitally entrapped in sodium alginate gel beads with its specific virulent bacteriophage EcP-01 to provide a new method for microbially digesting chitinous peritrophic membranes of phytophagous ladybird beetles Epilachna vigintioctopunctata. First, chitinase SH1 from a gram-positive bacterium Kurthia zopfii was overproduced by Escherichia coli cells and purified by affinity column chromatography. The purified enzyme effectively digested peritrophic membranes dissected from the ladybird beetles to expose epithelial tissues beneath the peritrophic membrane, and the beetles that had ingested chitinase after submergence in chitinase solution had considerably reduced their feeding on tomato leaves. KPM-007E/chi, entrapped in the alginate beads, released the chitinase. More chitinase was released when KPM-007E/chi was present with their specific virulent bacteriophage EcP-01 in the beads because of lysis of bacterial cells infected with the bacteriophages. This chitinase release from the microbial beads (containing KPM-007E/chi and EcP-01) was sufficient to digest the peritrophic membrane as well as to suppress feeding of bead-sprayed tomato leaves by the ladybird beetles. A daily supply of tomato leaves treated with the microbial beads considerably suppressed leaf feeding and oviposition by the ladybird beetles, suggesting a possible application of chitinase-secreting bacteria for suppressing herbivorous insect pests. |
| Databáze: | OpenAIRE |
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