Influence of severe endometriosis on gene expression of vascular endothelial growth factor and interleukin-6 in granulosa cells from patients undergoing controlled ovarian hyperstimulation for in vitro fertilization-embryo transfer

Autor: Yoshito Terai, Yao Ching Hung, Mikio Takehara, Yoshihiko Suzuki, Minoru Ueki, Masatsugu Ueda, Hikari Yamashita, Yoshiki Yamashita
Rok vydání: 2002
Předmět:
Vascular Endothelial Growth Factor A
Infertility
medicine.medical_specialty
Menotropins
Reproductive Techniques
Assisted
medicine.medical_treatment
Endometriosis
Gene Expression
Endothelial Growth Factors
Fertilization in Vitro
Controlled ovarian hyperstimulation
Biology
Chorionic Gonadotropin
Polymerase Chain Reaction
chemistry.chemical_compound
Ovulation Induction
Pregnancy
Internal medicine
medicine
Humans
Prospective Studies
Ovarian follicle
Progesterone
Lymphokines
Granulosa Cells
In vitro fertilisation
Estradiol
Interleukin-6
Vascular Endothelial Growth Factors
Obstetrics and Gynecology
Fallopian Tube Diseases
Embryo Transfer
medicine.disease
Embryo transfer
Vascular endothelial growth factor
Endocrinology
medicine.anatomical_structure
Reproductive Medicine
chemistry
Oocytes
Intercellular Signaling Peptides and Proteins
Female
Ovulation induction
Follicle Stimulating Hormone
Infertility
Female
Zdroj: Fertility and Sterility. 78:865-871
ISSN: 0015-0282
DOI: 10.1016/s0015-0282(02)03339-3
Popis: Objective: To evaluate how endometriosis affects expression of vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) in granulosa cells. Design: Prospective study. Setting: IVF-ET program at Osaka Medical College. Patient(s): Seventeen patients with revised American Fertility Society stage IV endometriosis and 17 patients with tubal infertility and no endometriosis. Intervention(s): Granulosa cells obtained at oocyte retrieval were examined for VEGF and IL-6 gene expression. Main Outcome Measure(s): Serum E 2 and P levels at hCG administration, number of oocytes, fertilization rate, high-quality embryo rate, and pregnancy rate, and expression of VEGF and IL-6 genes. Result(s): Total hMG and FSH levels were statistically significantly higher in patients with endometriosis; however, the number of retrieved oocytes and the fertilization rate were lower compared with patients with tubal infertility. Serum E 2 levels and expression of VEGF in patients with tubal infertility were statistically significantly higher than those in patients with endometriosis. Interleukin-6 gene expression did not differ between the groups. Conclusion(s): In severe endometriosis, lower VEGF gene expression in granulosa cells may adversely affect oocyte development and maturation.
Databáze: OpenAIRE
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