Presentation of Three Novel Tailed Phages Targeting Multiple Strains of Pseudomonas syringae
| Autor: | Horst Neve, Jacob Bruun Jørgensen, Alexander Byth Carstens, Witold Kot, Cindy E. Morris, Amaru Miranda Djurhuus, Lars Hestbjerg Hansen |
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| Přispěvatelé: | Department of Plant and Environmental Sciences [Copenhagen], Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Max Rubner-Institut, Unité de Pathologie Végétale (PV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), 'Phytoprotect' project funded by the Danish Research Council for Technology and Production Grants (Grant No. DFF-4184-00070), the Human Frontier Science Program (Grant No. RGP0024/2018), and the Villum Experiment (Grant No. 17595). |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
phage therapy Phage therapy medicine.medical_treatment viruses Biological pest control Pseudomonas syringae Microbiology Bacteriophage 03 medical and health sciences bacteriophage medicine biocontrol Pseudomonas phage 030304 developmental biology 0303 health sciences Agricultural plant biology Pseudomonas Phage 030306 microbiology fungi food and beverages Original Articles biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| Zdroj: | Phage (New Rochelle) Jørgensen, J B, Djurhuus, A M, Carstens, A B, Kot, W, Neve, H, Morris, C E & Hansen, L H 2020, ' Presentation of Three Novel Tailed Phages Targeting Multiple Strains of Pseudomonas syringae ', PHAGE: Therapy, Applications, and Research, vol. 1, no. 4, pp. 245-250 . https://doi.org/10.1089/phage.2020.0040 PHAGE PHAGE, 2020, 1 (4), pp.245-250. ⟨10.1089/phage.2020.0040⟩ |
| ISSN: | 2641-6530 |
| DOI: | 10.1089/phage.2020.0040 |
| Popis: | International audience; Background:Pseudomonas syringae are ubiquitous epiphytic plant pathogens infecting a wide range of important agricultural plant species. Bacteriophages has been proposed as biocontrol agents against plant pathogens, however, in order to utilize this approach, a deeper understanding of phage diversity and phage-host interactions is required.Materials and Methods: Phages targeting P. syringae GAW0113 were isolated from organic waste samples. Three distinct phage isolates were purified and subjected to whole-genome sequencing, comparative genomics, transmission electron microscopy and host-range assay using a wide selection of diverse P. syringae isolates.Results: The three phage isolates, Pseudomonas phage Bertil, Misse, and Strit, were shown to have podovirus morphology with a short tail stub and isometric head. They had double-stranded DNA ranging from 41,342 to 41,374 bp in size comprising 50–51 open reading frames. The three phage genomes were highly similar and genomic comparison analyses showed that they all belong to the Autographiviridae family of the order Caudovirales. All three phages were shown to have a narrow host-range.Conclusions: The three phages were shown to share morphological and genomic features with other phages in the Autographiviridae family, however, based on the limited nucleotide similarity we propose that the phages constitute a novel genus. All three phages were found to infect multiple strains of P. syringae covering several phylogroups. |
| Databáze: | OpenAIRE |
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