Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis

Autor: Rui-lian Jing, Li Qian, Ang Li, Xin-guo Mao, Jingyi Wang
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Sequence analysis
Transgene
Response element
drought tolerance
Arabidopsis
Flowers
Biology
01 natural sciences
Applied Microbiology and Biotechnology
Plant Roots
03 medical and health sciences
chemistry.chemical_compound
Abscisic acid
Protein Domains
Gene Expression Regulation
Plant
Sequence Analysis
Protein
Stress
Physiological
Botany
Gene expression
Molecular Biology
Transcription factor
Ecology
Evolution
Behavior and Systematics
Phylogeny
Triticum
Plant Proteins
Plant Stems
bZIP domain
food and beverages
Water
Cell Biology
biology.organism_classification
Plants
Genetically Modified
freezing tolerance
Cell biology
Cold Temperature
Plant Leaves
030104 developmental biology
Basic-Leucine Zipper Transcription Factors
Phenotype
chemistry
gene expression
Sequence Alignment
010606 plant biology & botany
Developmental Biology
Research Paper
Zdroj: International Journal of Biological Sciences
ISSN: 1449-2288
Popis: AREB (ABA response element binding) proteins in plants play direct regulatory roles in response to multiple stresses, but their functions in wheat (Triticum aestivum L.) are not clear. In the present study, TaAREB3, a new member of the AREB transcription factor family, was isolated from wheat. Sequence analysis showed that the TaAREB3 protein is composed of three parts, a conserved N-terminal, a variable M region, and a conserved C-terminal with a bZIP domain. It belongs to the group A subfamily of bZIP transcription factors. TaAREB3 was constitutively expressed in stems, leaves, florets, anthers, pistils, seeds, and most highly, in roots. TaAREB3 gene expression was induced with abscisic acid (ABA) and low temperature stress, and its protein was localized in the nucleus when transiently expressed in tobacco epidermal cells and stably expressed in transgenic Arabidopsis. TaAREB3 protein has transcriptional activation activity, and can bind to the ABRE cis-element in vitro. Overexpression of TaAREB3 in Arabidopsis not only enhanced ABA sensitivity, but also strengthened drought and freezing tolerances. TaAREB3 also activated RD29A, RD29B, COR15A, and COR47 by binding to their promoter regions in transgenic Arabidopsis. These results demonstrated that TaAREB3 plays an important role in drought and freezing tolerances in Arabidopsis.
Databáze: OpenAIRE
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