Activation of Myosin-I by Members of the Ste20p Protein Kinase Family
| Autor: | Cunle Wu, David Y. Thomas, Emilia Furmaniak-Kazmierczak, Ekkehard Leberer, Sheu-Fen Lee, Graham P. Côté |
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| Rok vydání: | 1996 |
| Předmět: |
Saccharomyces cerevisiae Proteins
macromolecular substances Myosins Protein Serine-Threonine Kinases Mitogen-activated protein kinase kinase Models Biological Biochemistry MAP2K7 Mice GTP-Binding Proteins pharmaceutical Animals Dictyostelium ASK1 c-Raf Phosphorylation Protein kinase A Molecular Biology Serine/threonine-specific protein kinase Mitogen-Activated Protein Kinase 3 MAP kinase kinase kinase biology Cyclin-dependent kinase 2 Intracellular Signaling Peptides and Proteins Cell Biology MAP Kinase Kinase Kinases Rats Cell biology Enzyme Activation p21-Activated Kinases Guanosine 5'-O-(3-Thiotriphosphate) Calcium-Calmodulin-Dependent Protein Kinases biology.protein Ca(2+) Mg(2+)-ATPase Mitogen-Activated Protein Kinases |
| Zdroj: | Journal of Biological Chemistry. 271:31787-31790 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.50.31787 |
| Popis: | The heavy chain of myosin-ID isolated from Dictyostelium was identified as an in vitro substrate for members of the Ste20p family of serine/threonine protein kinases which are thought to regulate conserved mitogen-activated protein kinase pathways. Yeast Ste20p and Cla4p and mammalian p21-activated protein kinase (PAK) phosphorylated the heavy chain to 0.5-0.6 mol of Pi/mol and stimulated the actin-dependent Mg2+-ATPase activity to an extent equivalent to that of the Ste20p-like myosin-I heavy chain kinase isolated from Dictyostelium. PAK purified from rat brain required GTPgammaS-Cdc42 to express full activity, whereas recombinant mouse mPAK3 fused to glutathione S-transferase and purified from bacteria, and Ste20p and Cla4p purified from yeast extracts were fully active without GTPgammaS-Cdc42. These results suggest, together with the high degree of structural and functional conservation of Ste20p family members and myosin-I isoforms, that myosin-I activation by Ste20p family protein kinases may contribute to the regulation of morphogenetic processes in organisms ranging from yeast to mammalian cells. |
| Databáze: | OpenAIRE |
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