Dual-Targeting Glycol Chitosan/Heparin-Decorated Polypyrrole Nanoparticle for Augmented Photothermal Thrombolytic Therapy
Autor: | Chih Yu Chiang, Shuo Hsiu Kuo, Jiashing Yu, Yu Jui Fan, Ting Yu Lu, Kuan Ting Liu, Pei Ru Jheng, Han Yun Hsieh, Er Yuan Chuang, Ching Li Tseng, Edgar Daniel Quiñones |
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Rok vydání: | 2021 |
Předmět: |
Male
Materials science Light Polymers medicine.medical_treatment Nanoparticle 02 engineering and technology Polypyrrole Fibrin 03 medical and health sciences chemistry.chemical_compound medicine Zeta potential Animals Pyrroles Thrombolytic Therapy General Materials Science Thrombus 030304 developmental biology Chitosan Mice Inbred ICR 0303 health sciences biology Heparin technology industry and agriculture Endothelial Cells Thrombosis Thrombolysis Phototherapy Photothermal therapy 021001 nanoscience & nanotechnology medicine.disease P-Selectin chemistry biology.protein Nanoparticles 0210 nano-technology Biomedical engineering medicine.drug |
Zdroj: | ACS Applied Materials & Interfaces. 13:10287-10300 |
ISSN: | 1944-8252 1944-8244 |
Popis: | Near-infrared (NIR)-light-modulated photothermal thrombolysis has been investigated to overcome the hemorrhage danger posed by clinical clot-busting substances. A long-standing issue in thrombosis fibrinolytics is the lack of lesion-specific therapy, which should not be ignored. Herein, a novel thrombolysis therapy using photothermal disintegration of a fibrin clot was explored through dual-targeting glycol chitosan/heparin-decorated polypyrrole nanoparticles (GCS-PPY-H NPs) to enhance thrombus delivery and thrombolytic therapeutic efficacy. GCS-PPY-H NPs can target acidic/P-selectin high-expression inflammatory endothelial cells/thrombus sites for initiating lesion-site-specific thrombolysis by hyperthermia using NIR irradiation. A significant fibrin clot-clearance rate was achieved with thrombolysis using dual-targeting/modality photothermal clot disintegration in vivo. The molecular level mechanisms of the developed nanoformulations and interface properties were determined using multiple surface specific analytical techniques, such as particle size distribution, zeta potential, electron microscopy, Fourier-transform infrared spectroscopy (FTIR), wavelength absorbance, photothermal, immunofluorescence, and histology. Owing to the augmented thrombus delivery of GCS-PPY-H NPs and swift treatment time, dual-targeting photothermal clot disintegration as a systematic treatment using GCS-PPY-H NPs can be effectively applied in thrombolysis. This novel approach possesses a promising future for thrombolytic treatment. |
Databáze: | OpenAIRE |
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