Exposed to Sulfamethoxazole induced hepatic lipid metabolism disorder and intestinal microbiota changes on zebrafish (Danio rerio)
| Autor: | Hongfeng Zhao, Mutang Zheng, Ying Zheng, Yufei Wang, Gang Wang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Sulfamethoxazole Physiology Health Toxicology and Mutagenesis Danio Gut flora Ecotoxicology urologic and male genital diseases Toxicology Biochemistry Microbiology chemistry.chemical_compound RNA Ribosomal 16S medicine Animals Zebrafish Fatty acid metabolism biology Sequence Analysis RNA Gene Expression Profiling Reproducibility of Results Cell Biology General Medicine Zebrafish Proteins Lipid Metabolism bacterial infections and mycoses biology.organism_classification medicine.disease female genital diseases and pregnancy complications Gastrointestinal Microbiome Metabolism disorder Gastrointestinal Tract Liver chemistry Vacuolization Liver function Dysbiosis Water Pollutants Chemical |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 253:109245 |
| ISSN: | 1532-0456 |
| Popis: | Antibiotics are widely used around the world. Pollution of Sulfamethoxazole (SMX) in water poses a great threat to aquatic life. In this study, the toxic effects of SMX on the liver were assessed through RNA sequencing analysis and 16S rRNA sequencing analysis was conducted to determine the influence of SMX on gut microbiota of zebrafish (Danio rerio). Adult male zebrafish were exposed to 0, 5, 90 and 450 μg/L of environmentally relevant concentrations of SMX for 21 days respectively. The results showed that the liver had severe histopathological damages including pyknotic nuclei, cytoplasmic hyalinization and vacuolization and deformed hepatocytes with loose cell-to-cell contact. Transcriptomic analysis revealed that liver function was seriously affected by SMX exposure. Meanwhile, SMX exposure significantly inhibited the expression of genes associated with fatty acid synthesis, oxidation and transport. Besides, exfoliated and dissolved epithelial cells were observed in the gut after SMX treatment. Although there was no significant change on richness and species diversity of intestinal microbial community, the relative abundance of phylum and genus of SMX treatments were significantly different from that of control group. The present study implied that SMX may cause potential health risks to fish through inducing histopathological damages, genetic expression alterations, disorder of fatty acid metabolism and intestinal microbiota dysbiosis. |
| Databáze: | OpenAIRE |
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