The Effect of Peptidoglycan Stimulation on Basophil-Mediated Atopic Responses During Pregnancy and in Newborns
| Autor: | Yi-Ping Xu, Gui-ying Shi, Wei-guo Xu, Ling Yang, Ying Xiong, Yin-Shi Guo |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Pulmonary and Respiratory Medicine medicine.medical_specialty Basophil Degranulation Test Peptidoglycan Basophil Ligands medicine.disease_cause Basophil degranulation Histamine Release chemistry.chemical_compound Allergen Pregnancy Internal medicine Hypersensitivity medicine Humans Immunology and Allergy Genetic Predisposition to Disease RNA Messenger Cells Cultured Interleukin 4 Dose-Response Relationship Drug business.industry Postpartum Period Infant Newborn Degranulation Infant Fetal Blood Asthma Immunity Innate Toll-Like Receptor 2 Basophils Pregnancy Complications medicine.anatomical_structure Endocrinology chemistry Pediatrics Perinatology and Child Health Immunology Cytokines Female Cytokine secretion Interleukin-4 business Histamine |
| Zdroj: | Journal of Asthma. 48:374-379 |
| ISSN: | 1532-4303 0277-0903 |
| DOI: | 10.3109/02770903.2011.563810 |
| Popis: | Toll-like receptor (TLR) molecules play critical roles in directing the course of atopic diseases by recognizing specific microbial products that activate immune effector cell function.We determined if basophils harvested from neonates genetically predisposed to atopic disease had different levels of TLR2 expression and determined whether putative TLR2 ligands mediated cytokine secretion.Blood samples were collected from 10 asthmatic and 12 healthy women and their newborns. Basophil histamine was measured using the human basophil degranulation test and TLR2 expression was determined using nucleic acid hybridization in situ and flow cytometry. IL-4 levels were quantified by ELISA following allergen stimulation.The basophil degranulation index (DI) in granulocytes harvested from peripheral blood of asthmatic women was assessed following stimulation with either peptidoglycan (PGN) or Dermatophagoides farinae (Df) extract. The DI was significantly higher in atopic women than in healthy controls. Basophils purified from the cord blood of neonates born to atopic mothers produced more IL-4 compared with basophils purified from children born to nonatopic controls. Finally, TLR2 expression at the protein and mRNA levels was upregulated in cord blood basophils from neonates born to mothers with asthma following stimulation with PGN but not Df.These data suggested that TLR2-mediated innate immune responses play a role in augmenting allergic reactions through the modulation of basophil cytokine secretion and histamine release. Microbial components may activate basophils through TLR2 (especially for genetically predisposed infants) to release cytokines associated with an increased incidence of allergic diseases. |
| Databáze: | OpenAIRE |
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