Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen
| Autor: | M J Siciliano, Daniel P. McCauliffe, Tsu-San Lieu, J Hanke, Y Itoh, M M Newkirk, M Reichlin, L L Bachinski, Ignacio Sanz, F A Lux |
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| Rok vydání: | 1990 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Gene Expression Biology Autoantigens Epitope Cell Line Chromosome 19 Sequence Homology Nucleic Acid Gene expression RNA Small Cytoplasmic Ultraviolet light Humans Amino Acid Sequence Cloning Molecular Gene Peptide sequence Gene Library Base Sequence Nucleic Acid Hybridization General Medicine DNA Blotting Northern Molecular biology Open reading frame Blotting Southern Ribonucleoproteins Human genome Oligonucleotide Probes Chromosomes Human Pair 19 Software Research Article |
| Zdroj: | The Journal of clinical investigation. 85(5) |
| ISSN: | 0021-9738 |
| Popis: | Ro/SS-A antibodies are found in a number of human autoimmune disorders including Sjogren's syndrome and several systemic lupus erythematosus-related disorders. These heterogeneous autoantibodies are known to recognize several distinct cellular antigens. With synthetic oligonucleotides corresponding to amino acid sequence information we have isolated a full-length cDNA clone which encodes a human Ro ribonucleoprotein autoantigen. The 1,890-base pair clone contains an open reading frame that encodes a 417-amino acid, 48-kD polypeptide that migrates aberrantly at 60 kD by SDS-PAGE. Rabbit antibodies raised against this protein's recently described amino-terminal epitope react with a previously identified 52-kD human Ro protein and immunoprecipitate the human cytoplasmic RNAs. Ultraviolet light cross-linking studies suggest that this Ro protein binds each of the four major human cytoplasmic RNAs. The deduced amino acid sequence is 63% homologous to an Onchocerca volvulus antigen. Southern filter hybridization analysis indicates that this gene is not highly polymorphic and exists as a single copy in the human genome. Chromosomal localization studies place this gene on the short arm of chromosome 19 near the gene encoding the low density lipoprotein receptor. |
| Databáze: | OpenAIRE |
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