Quantification of Human Plasma Apolipoproteins C-I, C-II, and C-III by Radioimmunoassays
| Autor: | Amita Rastogi, Nina Bren, Bruce A. Kottke |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Adult
Male medicine.medical_specialty Radioimmunoassay Hyperlipoproteinemia Type V Hyperlipoproteinemia Type IV Sensitivity and Specificity Diabetes mellitus Internal medicine Blood plasma Diabetes Mellitus medicine Humans Apolipoproteins C Aged Apolipoprotein C-I Apolipoprotein C-III Chromatography Chemistry Hypertriglyceridemia Reproducibility of Results Fast protein liquid chromatography Liter General Medicine Middle Aged medicine.disease Endocrinology Human plasma Diethylaminoethyl cellulose Apolipoprotein C-II Female lipids (amino acids peptides and proteins) |
| Zdroj: | Mayo Clinic Proceedings. 68:657-664 |
| ISSN: | 0025-6196 |
| DOI: | 10.1016/s0025-6196(12)60601-6 |
| Popis: | We have developed radioimmunoassays for the quantification of apolipoproteins (apo) C-I, C-II, and C-III in human plasma. The apo C proteins were isolated from very low-density lipoproteins of patients with hypertriglyceridemia, fractionated on a Sephacryl column, and purified by diethylaminoethyl cellulose anion-exchange chromatography followed by reverse-phase fast protein liquid chromatography. The assays were sensitive, specific, and reproducible, and the standards demonstrated parallel immunoreactivity with plasma samples. Patients with hypertriglyceridemia (triglyceride level more than 2,200 mg/liter)--14 patients with diabetes and 12 with type V hyperlipoproteinemia--were compared with age- and sex-matched control subjects. In comparison with the control groups, levels of apoproteins C-I, C-II, and C-III were significantly increased in both disease groups, but the ratios of the C peptides to triglycerides were significantly lower, an indication of a relative deficiency of C apoproteins in hypertriglyceridemic states. Independent radioimmunoassays for each of the C apolipoproteins would help to study their individual roles in triglyceride-rich lipoprotein metabolism. |
| Databáze: | OpenAIRE |
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