Targeted Delivery of Miconazole Employing LL37 Fragment Mutant Peptide CKR12-Poly (Lactic-Co-Glycolic) Acid Polymeric Micelles

Autor:	Toshihiro Nagao, Takahiro Uchida, Daisuke Ishibashi, Rie Kakehashi, Miki Yoshii, Mai Hazekawa, Yoshiro Hatanaka, Makoto Nakagawa, Takeshi Mori, Honami Kojima, Miyako Yoshida, Rio Uno
Rok vydání:	2021
Předmět:	Antifungal Agents antimicrobial peptide Miconazole QH301-705.5 medicine.medical_treatment Antimicrobial peptides Peptide Micelle Catalysis Article Cathelicidin Inorganic Chemistry chemistry.chemical_compound Drug Delivery Systems Polylactic Acid-Polyglycolic Acid Copolymer Cathelicidins transmission electron microscopy micelle Candida albicans medicine Physical and Theoretical Chemistry Biology (General) Molecular Biology QD1-999 Spectroscopy Micelles chemistry.chemical_classification Chemistry Organic Chemistry technology industry and agriculture Candidiasis drug targeting General Medicine Antimicrobial Combinatorial chemistry Computer Science Applications PLGA Targeted drug delivery Drug delivery drug delivery Nanoparticles scanning electron microscopy Antimicrobial Cationic Peptides
Zdroj:	International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 12056, p 12056 (2021) Volume 22 Issue 21
ISSN:	1422-0067
Popis:	We previously reported that conjugates of antimicrobial peptide fragment analogues and poly (lactic-co-glycolic) acid (PLGA) enhance antimicrobial activity and that the conjugated micelle structure is an effective tool for antimicrobial drug delivery. In recent years, the delivery of antimicrobial peptides to targets for antimicrobial activity has attracted attention. In this study, we targeted Candida albicans, a causative organism of catheter-related bloodstream infections, which is refractory to antimicrobial agents and is currently a problem in medical practice. We evaluated the antifungal activity of CKR12 (a mutant fragment of the human cathelicidin peptide, LL-37)-PLGA-miconazole (MCZ) micelles using nanotechnology with MCZ delivery. The prepared CKR12-PLGA-MCZ micelles were characterised by measuring dynamic light scattering, zeta potential, dilution stability, and drug release. CKR12-PLGA-MCZ micelles showed higher antifungal activity than CKR12-PLGA micelles and MCZ solution. Furthermore, scanning and transmission electron microscopy suggested that CKR12-PLGA-MCZ micelles disrupted both cell wall and cell membrane of C. albicans. Our results revealed a synergistic effect of antifungal activity using a combination of antimicrobial peptide fragment analogues and MCZ, and that MCZ is a promising tool for the delivery to target microorganisms.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22df2ef713e70e5edc68c473dc3222a5 https://pubmed.ncbi.nlm.nih.gov/34769486 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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