Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus
| Autor: | Dragica Jakovljević, Anita Klaus, Johannes P. F. G. Helsper, Miomir Nikšić, L.J.L.D. van Griensven, Maja Kozarski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
fruiting bodies Immunomodulating activity water phenols edible mushrooms tyrosinase Polysaccharide 01 natural sciences PRI BIOS Applied Genomics & Proteomics Analytical Chemistry Microbiology chemistry.chemical_compound 0404 agricultural biotechnology Antioxidant activity Polysaccharides 010608 biotechnology Phenols Food science Ethanol precipitation EC50 antitumor chemistry.chemical_classification biology monophenols 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science Spore Hot water extraction chemistry Phellinus linteus glucans Medicinal mushrooms BIOS Applied Metabolic Systems interferon-gamma Agaricus bisporus Food Science |
| Zdroj: | Food Chemistry, 129(4), 1667-1675 Food Chemistry Food Chemistry 129 (2011) 4 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2011.06.029 |
| Popis: | Partially purified polysaccharides were obtained from four medicinal mushroom species, Agaricus bisporus, Agaricus brasiliensis, Phellinus linteus and Ganoderma lucidum by hot water extraction, followed by ethanol precipitation. The four samples contained varying amounts of both alpha- and beta-glucans as determined by FT-IR and by quantitative estimation after prior partial hydrolysis (Megazyme beta-glucan assay kit). EC50 values of the DPPH scavenging activity of the polysaccharides from G. lucidum spores and P. linteus fruiting bodies were found to be particularly low, i.e. EC50 LT 0.1 mg/ml. For A. brasiliensis and A. bisporus, EC50 values were 0.27 and 2.0 mg/ml. EC50 values of the antioxidant activity were 7.07 mg/ml for G. lucidum, 13.25 mg/ml for A. brasiliensis and >20 mg/ml for A. bisporus polysaccharide, respectively. EC50 values of the chelating activity of ferrous ions ranged from 0.59 mg/ml for G. lucidum to 7.80 mg/ml for A. bisporus. The EC50 values of the extracts in the reducing power assay ranged from 0.47 to 14.83 mg/ml. A correlation was found between EC50 values of the chelating and reducing power abilities and the amount of total glucans content in the extracts. In vitro measurements of immunomodulatory capacity of polysaccharide extracts showed that A. bisporus, A. brasiliensis fruiting bodies and G. lucidum spores extracts express an immunostimulating effect on activated human PBMCs and induce synthesis of IFN-gamma. The polysaccharide extract of P. linteus fruiting bodies showed an immunosuppressive effect. |
| Databáze: | OpenAIRE |
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