A proteomic analysis of the statocyst endolymph in common cuttlefish (Sepia officinalis): an assessment of acoustic trauma after exposure to sound

Autor: Michel André, Marta Monge, Carmen Quero, Marta Solé
Přispěvatelé: Quero, Carmen [0000-0003-3599-2778], Centre Tecnològic de Vilanova i la Geltrú, Universitat Politècnica de Catalunya. LAB - Laboratori d'Aplicacions Bioacústiques, Quero, Carmen
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Física::Acústica::Sons subaquàtics [Àrees temàtiques de la UPC]
0301 basic medicine
Cuttlefish
So
Proteomics
Proteome
Endolymph
lcsh:Medicine
Epithelium
Environmental impact
Sound exposure
0302 clinical medicine
Electrophoresis
Gel
Two-Dimensional
Sepia
lcsh:Science
Cytoskeleton
Multidisciplinary
biology
Ecology
Decapodiformes
Statocyst endolymph
Cell biology
medicine.anatomical_structure
Sound
Cefalòpodes
Acoustic trauma
Epiteli
Cephalopods
Soroll -- Aspectes ambientals
Context (language use)
Marine pollution
Noise barriers
Article
03 medical and health sciences
Bioacústica
medicine
otorhinolaryngologic diseases
Animals
Ciències de la salut::Impacte ambiental [Àrees temàtiques de la UPC]
lcsh:R
Environmental Exposure
Contaminació acústica marina
biology.organism_classification
Statocyst
Cephalopod
030104 developmental biology
Environmental impact analysis
Cephalopoda
lcsh:Q
sense organs
Barreres acústiques
Bioacoustics
030217 neurology & neurosurgery
Zdroj: Scientific Reports
Dipòsit Digital de la UB
Universidad de Barcelona
Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
UPCommons. Portal del coneixement obert de la UPC
Universitat Politècnica de Catalunya (UPC)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Recercat. Dipósit de la Recerca de Catalunya
ISSN: 2045-2322
Popis: Recent studies, both in laboratory and sea conditions, have demonstrated damage after sound exposure in the cephalopod statocyst sensory epithelium, which secretes endolymph protein. Here, the proteomic analysis of the endolymph was performed before and after sound exposure to assess the effects of exposure to low intensity, low frequency sounds on the statocyst endolymph of the Mediterranean common cuttlefish (Sepia officinalis), determining changes in the protein composition of the statocyst endolymph immediately and 24 h after sound exposure. Significant differences in protein expression were observed, especially 24 h after exposure. A total of 37 spots were significantly different in exposed specimens, 17 of which were mostly related to stress and cytoskeletal structure. Among the stress proteins eight spots corresponding to eight hemocyanin isoforms were under-expressed possible due to lower oxygen consumption. In addition, cytoskeletal proteins such as tubulin alpha chain and intermediate filament protein were also down-regulated after exposure. Thus, endolymph analysis in the context of acoustic stress allowed us to establish the effects at the proteome level and identify the proteins that are particularly sensitive to this type of trauma.
We thank E. Escolar of the vessel Nova Míriam for assisting in the collection of cephalopod specimens and A. Guerrero for valuable comments on the paper.
Databáze: OpenAIRE
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