Panax ginseng induces the expression of CatSper genes and sperm hyperactivation
| Autor: | Seong Kyu Park, Ha Young Kim, Do Rim Kim, Mun Seog Chang, Eun Hwa Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Urology Panax Ca 2 + CatSper hyperactivation panax ginseng Biology Semen analysis lcsh:RC870-923 Andrology Ginseng Mice Gene expression medicine Animals Gene Sperm motility Hyperactivation medicine.diagnostic_test Traditional medicine urogenital system Plant Extracts General Medicine lcsh:Diseases of the genitourinary system. Urology Sperm Spermatozoa Blot Mice Inbred C57BL Ca2+ Gene Expression Regulation Original Article Calcium Channels |
| Zdroj: | Asian Journal of Andrology Asian Journal of Andrology, Vol 16, Iss 6, Pp 845-851 (2014) |
| ISSN: | 1745-7262 1008-682X |
| Popis: | The cation channel of sperm ( CatSper ) protein family plays important roles in male reproduction and infertility. The four members of this family are expressed exclusively in the testis and are localized differently in sperm. To investigate the effects of Panax ginseng treatment on the expression of CatSper genes and sperm hyperactivation in male mice, sperm motility and CatSper gene expression were assessed using a computer-assisted semen analysis system, a Fluoroskan Ascent microplate fluorometer to assess Ca 2+ influx, real-time polymerase chain reaction, Western blotting and immunofluorescence. The results suggested that the Ca 2+ levels of sperm cells treated with P. ginseng were increased significantly compared with the normal group. The P. ginseng -treated groups showed increased sperm motility parameters, such as the curvilinear velocity and amplitude of lateral head displacement. Taken together, the data suggest that CatSper messenger ribonucleic acid levels were increased significantly in mouse testes in the P. ginseng - treated group, as was the protein level, with the exception of CatSper 2. In conclusion, P. ginseng plays an important role in improving sperm hyperactivation via CatSper gene expression. |
| Databáze: | OpenAIRE |
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