Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue
| Autor: | N. V. Kabaeva, Yu. A. Romanov, E. E. Balashova, N E Volgina, Gennady T. Sukhikh, T. N. Dugina |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Culture Techniques Biology Mesenchymal Stem Cell Transplantation Umbilical cord Regenerative medicine General Biochemistry Genetics and Molecular Biology Cryopreservation Umbilical Cord 03 medical and health sciences Stroma medicine Humans Autologous transplantation Cells Cultured Cell Proliferation Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Cord lining Cell biology 030104 developmental biology medicine.anatomical_structure Cell culture Female |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 160:530-534 |
| ISSN: | 1573-8221 0007-4888 |
| Popis: | Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies. |
| Databáze: | OpenAIRE |
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