Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System
| Autor: | I. Darah, M.M.J. Taufiq |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
ocimum sanctum linn
endophytic fungus submerged fermentation Anti mrsa Endophytic fungus Biology Applied Microbiology and Biotechnology Microbiology Submerged fermentation QR1-502 Horticulture lasiodiplodia pseudotheobromae ibrl os-64 Ocimum sanctum L anti-mrsa activity Lasiodiplodia pseudotheobromae Biotechnology |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 13, Iss 4, Pp 2517-2531 (2019) |
| ISSN: | 2581-690X 0973-7510 |
| DOI: | 10.22207/jpam.13.4.67 |
| Popis: | The effects of cultural conditions on the production of anti-MRSA activity of the endophytic fungal isolate, Lasiodiplodia pseudotheobromae IBRL OS-64 isolated from the leaf of a local medicinal herb, Ocimum sanctum Linn. was studied. The highest anti-MRSA activity of 50.0±0.1 U/mL and 1.46±0.1 g/L of fungal growth were obtained after incorporating all the improved cultural conditions which consisted of yeast extract sucrose broth supplemented with host plant extract, with initial medium pH of 6, 2 mycelial plugs of 4 days old seed culture, cultivation temperature of 30°C and cultivated for 16 days under static conditions without the presence of light. After improvement of cultural conditions, a significant increment of anti-MRSA activity of about 57.57% (2.4 folds) and a slight increment of fungal growth of about 8.15% (1.1 folds) were obtained compared to the cultural condition before improvement. Indeed the improvement of cultural conditions greatly affected the anti-MRSA activity and growth production by L. pseudotheobromae IBRL OS-64 isolate. |
| Databáze: | OpenAIRE |
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