Pathogen challenge, salicylic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine
| Autor: | Jon Reed, Janice E. K. Cooke, John M. Davis, Charles H. Michler, Haiguo Wu, K. Scott Luce |
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| Rok vydání: | 2002 |
| Předmět: |
Genotype
Physiology Molecular Sequence Data Sequence Homology Cyclopentanes Biology Gene Expression Regulation Enzymologic chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Gene expression Tobacco Extracellular Escherichia coli Amino Acid Sequence Oxylipins Cloning Molecular Pathogenesis-related protein Plant Diseases Jasmonic acid Chitinases Fungi General Medicine biology.organism_classification Pinus Elicitor Fusarium subglutinans chemistry Biochemistry Multigene Family Chitinase biology.protein Salicylic Acid Agronomy and Crop Science Salicylic acid |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 15(4) |
| ISSN: | 0894-0282 |
| Popis: | To better understand the molecular regulation of defense responses in members of the genus Pinus, we tested the expression of various chitinase homologs in response to pathogen-associated signals. PSCHI4, a putative extracellular class II chitinase, was secreted into liquid medium by pine cells and was also secreted by transgenic tobacco cells that ectopically expressed pschi4. Extracellular proteins of pine were separated by isoelectric focusing; PSCHI4 was not associated with fractions containing detectable β-N-acetylglucosaminidase or lysozyme activities. However, other fractions contained enzyme activities that increased markedly after elicitor treatment. The pschi4 transcript and protein accumulated in pine seedlings challenged with the necrotrophic pathogen Fusarium subglutinans f. sp. pini, with the protein reaching detectable levels in susceptible seedlings concomitant with the onset of visible disease symptoms. Additional chitinase transcripts, assigned to classes I and IV based on primary sequence analysis, were also induced by pathogen challenge. Jasmonic acid induced class I and class IV but not class II chitinase, whereas salicylic acid induced all three classes of chitinase. These results show that multiple chitinase homologs are induced after challenge by a necrotrophic pathogen and by potential signaling molecules identified in angiosperms. This suggests the potential importance of de novo pathogenesis-related (PR) gene expression in pathogen defense responses of pine trees. |
| Databáze: | OpenAIRE |
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