Effect of administering a crude equine gonadotrophin preparation to mares on follicular development, oocyte recovery rate and oocyte maturation in vivo
| Autor: | B. Synnestvedt, M Baltsen, G Duchamp, I. Brück, Torben Greve, I Couty, J. Bezard |
|---|---|
| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration |
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
endocrine system Embryology Gonadotropins Equine 040301 veterinary sciences medicine.medical_treatment [SDV]Life Sciences [q-bio] Fertilization in Vitro [INFO] Computer Science [cs] Biology 0403 veterinary science 03 medical and health sciences Oogenesis 0302 clinical medicine Endocrinology Estrus Ovarian Follicle Internal medicine Follicular phase medicine Animals HORMONE GONADOTROPE [INFO]Computer Science [cs] Horses Ovarian follicle ComputingMilieux_MISCELLANEOUS In vitro fertilisation Germinal vesicle 030219 obstetrics & reproductive medicine 0402 animal and dairy science Obstetrics and Gynecology 04 agricultural and veterinary sciences Cell Biology Oocyte Cumulus oophorus Follicular fluid 040201 dairy & animal science Follicular Fluid In vitro maturation [SDV] Life Sciences [q-bio] Meiosis Sterols medicine.anatomical_structure Reproductive Medicine Oocytes Tissue and Organ Harvesting Female |
| Zdroj: | Journal of Reproduction and Fertility Journal of Reproduction and Fertility, Portland Press, 2000, 118, pp.351-360 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/reprod/118.2.351 |
| Popis: | In mares, the shortage of oocytes and the variability in nuclear maturation at a certain time of the oestrous cycle hinders the optimization of methods for in vitro maturation and in vitro fertilization. Increasing the number of small-to-medium-sized follicles available for aspiration in vivo may increase the overall oocyte yield. The aims of the present study were to investigate whether administration of crude equine gonadotrophins affects follicular development, oocyte recovery rate, in vivo oocyte maturation and follicular concentrations of meiosis-activating sterols. During oestrus, all follicles >/= 4 mm were aspirated from 19 pony mares (first aspiration: A1). Over the next 8 days, the mares were treated daily with either 25 mg crude equine gonadotrophins (n = 10) or physiological saline (n = 9). Between day 1 and day 8, follicular growth was monitored by ultrasonography. On day 8, all follicles >/= 4 mm were evacuated (second aspiration: A2) and nuclear maturation of the recovered oocytes was assessed after orcein staining. Follicular growth between A1 and A2, as well as the number and size of follicles at A2 were similar for control mares and mares treated with crude equine gonadotrophins. The oocyte recovery rates at A1 and A2 were similar. At A2, the oocyte recovery rate and oocyte maturation in vivo were not affected by treatment with crude equine gonadotrophins. The number of expanded cumulus oophorus complexes recovered from follicles |
| Databáze: | OpenAIRE |
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