miR‑590‑5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines
| Autor: | Xiushan Wu, Yao Wen, Yu Chen, Wuzhou Yuan, Zhigang Jiang, Yongqi Wan, Xiongwei Fan, Fang Li, Sixing Chen, Yuequn Wang, Ping Zhu, Wanwan Cai, Yongqing Li, Jian Zhuang |
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| Rok vydání: | 2021 |
| Předmět: |
Cancer Research
cell migration biology Oncogene pancreatic cancer Cell Cell migration Articles microRNA-590-5p Cell cycle Meiotic nuclear division required for meiotic nuclear division 5 homolog A Ubiquitin ligase Gene expression profiling medicine.anatomical_structure Oncology microRNA biology.protein Cancer research medicine prognosis |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2021.12793 |
| Popis: | Required for meiotic nuclear division 5 homolog A (RMND5A) functions as an E3 ubiquitin ligase. To date, few studies have investigated the role of RMND5A in cancer. In the present study, the expression levels of RMND5A in multiple types of cancer were analyzed using the Gene Expression Profiling Interactive Analysis platform. The results revealed that RMND5A was highly expressed and associated with overall survival in patients with pancreatic adenocarcinoma (PAAD). A wound-healing assay revealed that RMND5A overexpression significantly increased cell migration in the PAAD cell lines AsPC-1 and PANC-1. In silico analysis predicted that RMND5A was a potential target of microRNA(miR)-590-5p. Further in vitro experiments demonstrated that overexpression of miR-590-5p downregulated the expression levels of RMND5A and decreased the migratory ability of the AsPC-1 and PANC-1 cell lines. In addition, overexpression of miR-590-5p attenuated the promoting effects of RMND5A on the migration of AsPC-1 and PANC-1 cells. The results of the present study may further elucidate the mechanisms underlying PAAD progression and provide novel targets for the treatment of PAAD. |
| Databáze: | OpenAIRE |
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