Cell-type- and tissue-specific transcriptomes of the white spruce (Picea glauca ) bark unmask fine-scale spatial patterns of constitutive and induced conifer defense
| Autor: | Karen E. Reid, Jose M. Celedon, Jörg Bohlmann, Angela Chiang, Hannah Henderson, Macaire M.S. Yuen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecta RNA-Seq Cyclopentanes Laser Capture Microdissection Plant Science Acetates Phloem Biology 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Botany Genetics Plant Bark Plant defense against herbivory Animals Cluster Analysis Oxylipins Picea Disease Resistance Plant Diseases Plant Proteins Laser capture microdissection Methyl jasmonate Sequence Analysis RNA Terpenes fungi Cell Biology 15. Life on land Cell biology 030104 developmental biology chemistry Organ Specificity visual_art visual_art.visual_art_medium Bark 010606 plant biology & botany |
| Zdroj: | The Plant Journal. 92:710-726 |
| ISSN: | 0960-7412 |
| Popis: | Plant defenses often involve specialized cells and tissues. In conifers, specialized cells of the bark are important for defense against insects and pathogens. Using laser microdissection, we characterized the transcriptomes of cortical resin duct cells, phenolic cells and phloem of white spruce (Picea glauca) bark under constitutive and methyl jasmonate (MeJa)-induced conditions, and we compared these transcriptomes with the transcriptome of the bark tissue complex. Overall, ~3700 bark transcripts were differentially expressed in response to MeJa. Approximately 25% of transcripts were expressed in only one cell type, revealing cell specialization at the transcriptome level. MeJa caused cell-type-specific transcriptome responses and changed the overall patterns of cell-type-specific transcript accumulation. Comparison of transcriptomes of the conifer bark tissue complex and specialized cells resolved a masking effect inherent to transcriptome analysis of complex tissues, and showed the actual cell-type-specific transcriptome signatures. Characterization of cell-type-specific transcriptomes is critical to reveal the dynamic patterns of spatial and temporal display of constitutive and induced defense systems in a complex plant tissue or organ. This was demonstrated with the improved resolution of spatially restricted expression of sets of genes of secondary metabolism in the specialized cell types. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text