Changes in synaptic structure underlie the developmental speeding of AMPA receptor–mediated EPSCs

Autor: Stuart G. Cull-Candy, David A. DiGregorio, Laurence Cathala, Zoltan Nusser, Noemi Holderith
Rok vydání: 2005
Předmět:
Patch-Clamp Techniques
Postsynaptic Current
Models
Neurological
Glutamic Acid
AMPA receptor
In Vitro Techniques
Neurotransmission
Biology
Kynurenic Acid
Synaptic Transmission
Membrane Potentials
Synapse
Benzodiazepines
Mice
Imaging
Three-Dimensional
Nerve Fibers
Microscopy
Electron
Transmission
Cerebellum
Neuropil
medicine
Animals
Receptors
AMPA
Ultrasonography
Neurons
musculoskeletal
neural
and ocular physiology
General Neuroscience
Age Factors
Electric Conductivity
Temperature
Glutamate receptor
Excitatory Postsynaptic Potentials
Dose-Response Relationship
Radiation
Immunohistochemistry
Electric Stimulation
Mice
Inbred C57BL
medicine.anatomical_structure
Animals
Newborn
nervous system
Synapses
Silent synapse
Excitatory postsynaptic potential
sense organs
Excitatory Amino Acid Antagonists
Neuroscience
Zdroj: Nature Neuroscience. 8:1310-1318
ISSN: 1546-1726
1097-6256
DOI: 10.1038/nn1534
Popis: At many excitatory and inhibitory synapses throughout the nervous system, postsynaptic currents become faster as the synapse matures, primarily owing to changes in receptor subunit composition. The origin of the developmental acceleration of AMPA receptor (AMPAR)-mediated excitatory postsynaptic currents (EPSCs) remains elusive. We used patch-clamp recordings, electron microscopic immunogold localization of AMPARs, partial three-dimensional reconstruction of the neuropil and numerical simulations of glutamate diffusion and AMPAR activation to examine the factors underlying the developmental speeding of miniature EPSCs in mouse cerebellar granule cells. We found that the main developmental change that permits submillisecond transmission at mature synapses is an alteration in the glutamate concentration waveform as experienced by AMPARs. This can be accounted for by changes in the synaptic structure and surrounding neuropil, rather than by a change in AMPAR properties. Our findings raise the possibility that structural alterations could be a general mechanism underlying the change in the time course of AMPAR-mediated synaptic transmission.
Databáze: OpenAIRE
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