Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation
| Autor: | Tore Bengtsson, Didier F. Pisani, Dana S. Hutchinson, Jean-Claude Chambard, Anna L. Sandström, Ez-Zoubir Amri, Jessica M. Olsen, Masaaki Sato, Olof S. Dallner |
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| Přispěvatelé: | Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA) |
| Rok vydání: | 2014 |
| Předmět: |
Male
Glucose uptake Adipose tissue MESH: Isoproterenol Mice 0302 clinical medicine Brown adipose tissue Immunology and Allergy Glucose homeostasis Insulin MESH: Animals Phosphorylation [SDV.BDD]Life Sciences [q-bio]/Development Biology Cells Cultured Research Articles MESH: Adipocytes Brown 0303 health sciences Glucose Transporter Type 1 biology TOR Serine-Threonine Kinases MESH: Adrenergic beta-3 Receptor Agonists 3. Good health MESH: Glucose Protein Transport medicine.anatomical_structure Adipocytes Brown Biochemistry Female hormones hormone substitutes and hormone antagonists MESH: Cells Cultured MESH: Protein Transport Snf3 endocrine system Morpholines Immunology Primary Cell Culture MESH: Morpholines Adrenergic beta-3 Receptor Agonists MESH: Insulin Mechanistic Target of Rapamycin Complex 2 Article MESH: Primary Cell Culture 03 medical and health sciences medicine Animals Humans MESH: Mice MESH: TOR Serine-Threonine Kinases PI3K/AKT/mTOR pathway 030304 developmental biology Sirolimus MESH: Humans MESH: Phosphorylation Multipotent Stem Cells Isoproterenol nutritional and metabolic diseases Cell Biology MESH: Multiprotein Complexes MESH: Male carbohydrates (lipids) Glucose Pyrimidines MESH: Protein Processing Post-Translational MESH: Pyrimidines Multiprotein Complexes Receptors Adrenergic beta-3 biology.protein GLUT1 MESH: Sirolimus MESH: Multipotent Stem Cells MESH: Receptors Adrenergic beta-3 MESH: Female Protein Processing Post-Translational 030217 neurology & neurosurgery MESH: Glucose Transporter Type 1 |
| Zdroj: | The Journal of Cell Biology Journal of Cell Biology Journal of Cell Biology, Rockefeller University Press, 2014, 207 (3), pp.365-74 |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | β3-Adrenoceptors promote glucose uptake in brown adipose tissue via both cAMP-mediated increases in GLUT1 transcription and mTORC2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane. Brown adipose tissue is the primary site for thermogenesis and can consume, in addition to free fatty acids, a very high amount of glucose from the blood, which can both acutely and chronically affect glucose homeostasis. Here, we show that mechanistic target of rapamycin (mTOR) complex 2 has a novel role in β3-adrenoceptor–stimulated glucose uptake in brown adipose tissue. We show that β3-adrenoceptors stimulate glucose uptake in brown adipose tissue via a signaling pathway that is comprised of two different parts: one part dependent on cAMP-mediated increases in GLUT1 transcription and de novo synthesis of GLUT1 and another part dependent on mTOR complex 2–stimulated translocation of newly synthesized GLUT1 to the plasma membrane, leading to increased glucose uptake. Both parts are essential for β3-adrenoceptor–stimulated glucose uptake. Importantly, the effect of β3-adrenoceptor on mTOR complex 2 is independent of the classical insulin–phosphoinositide 3-kinase–Akt pathway, highlighting a novel mechanism of mTOR complex 2 activation. |
| Databáze: | OpenAIRE |
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