Molecular basis of cell migration in the fish lateral line: Role of the chemokine receptor CXCR4 and of its ligand, SDF1
| Autor: | Christine Thisse, Laure Saint-Etienne, Alain Ghysen, Nicolas B. David, Frédéric M. Rosa, Christine Dambly-Chaudière, Dora Sapède, Bernard Thisse |
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| Přispěvatelé: | Génétique moléculaire du développement, Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-IFR36-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de neurogénétique, Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR122, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure - Paris (ENS Paris)-IFR36-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université Louis Pasteur - Strasbourg I, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2002 |
| Předmět: |
Receptors
CXCR4 MESH: Axons animal structures Neurite MESH: Embryonic Induction Lateral line Biology MESH: Receptors CXCR4 03 medical and health sciences Chemokine receptor Cell Movement Neurites Animals Cell Lineage MESH: Animals MESH: Sense Organs Primordium Neurons Afferent MESH: Zebrafish Lymphocyte homing receptor MESH: Cell Movement [SDV.BDD]Life Sciences [q-bio]/Development Biology Zebrafish MESH: Chemokines CXC 030304 developmental biology Embryonic Induction 0303 health sciences Multidisciplinary MESH: Neurons Afferent 030302 biochemistry & molecular biology Sense Organs Cell migration Anatomy Biological Sciences MESH: Cell Lineage biology.organism_classification MESH: Neurites Axons Chemokine CXCL12 Cell biology MESH: Chemokine CXCL12 Chemokines CXC |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2002, 99 (25), pp.16297-302. ⟨10.1073/pnas.252339399⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.252339399 |
| Popis: | Cell migration plays an essential role in many morphogenetic processes, and its deregulation has many dramatic consequences. Yet how migration is controlled during normal development is still a largely unresolved question. We examined this process in the case of the posterior lateral line (PLL), a mechanosensory system present in fish and amphibians. In zebrafish, the embryonic PLL comprises seven to eight sense organs (neuromasts) aligned from head to tail along the flank of the animal and is formed by a primordium that originates from a cephalic placode. This primordium migrates along a stereotyped pathway toward the tip of the tail and deposits in its wake discrete groups of cells, each of which will become a neuromast. We show that a trail of SDF1-like chemokine is present along the pathway of the primordium and that a CXCR4-like chemokine receptor is expressed by the migrating cells. The inactivation of either the ligand or its receptor blocks migration, whereas in mutants in which the normal SDF1 trail is absent, the primordium path is redirected to the next, more ventral sdf1 expression domain. In all cases, the sensory axons remain associated to the primordium, indicating that the extension of the neurites to form the PLL nerve depends on the movement of the primordium. We conclude that both the formation and the innervation of this system depend on the SDF1-CXCR4 system, which has also been implicated in several migration events in humans, including metastasis formation and lymphocyte homing. |
| Databáze: | OpenAIRE |
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