Alterations in B-cell subsets in pediatric patients with early atopic dermatitis
| Autor: | Hitokazu Esaki, Tali Czarnowicki, Sreya Talasila, Hui Xu, Xiuzhong Zheng, Emma Guttman-Yassky, Hugh A. Sampson, James G. Krueger, Mayte Suárez-Fariñas, T. Huynh, Yael Renert-Yuval, Isabel Haugh, Patrick M. Brunner, Yeriel Estrada, Gary Tran, Juana Gonzalez, Margeaux Oliva, Amy S. Paller, Sarah Lyon |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Aging Lymphocyte T-Lymphocytes Immunology B-Lymphocyte Subsets CD38 Immunoglobulin E Immunoglobulin D Dermatitis Atopic 03 medical and health sciences 0302 clinical medicine Antigen medicine Immunology and Allergy Humans SCORAD B cell Skin medicine.diagnostic_test biology business.industry Infant Atopic dermatitis Allergens Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure Child Preschool biology.protein Female business 030215 immunology |
| Zdroj: | The Journal of allergy and clinical immunology. 140(1) |
| ISSN: | 1097-6825 |
| Popis: | Background B cells undergo maturation and class-switching in response to antigen exposure and T-cell help. Early B-cell differentiation has not been defined in patients with early-onset atopic dermatitis (AD). Objective We sought to define the frequency of B-cell subsets associated with progressive B-cell maturation and IgE class-switching. Methods We studied 27 children and 34 adults with moderate-to-severe AD (mean SCORAD score, 55 and 65, respectively) and age-matched control subjects (15 children and 27 adults). IgD/CD27 and CD24/CD38 core gating systems and an 11-color flow cytometric panel were used to determine the frequencies of circulating B-cell subsets. Serum total and allergen-specific IgE (sIgE) levels were measured by using ImmunoCAP. Results Compared with adults, children showed T-cell predominance in the skin. Circulating CD19 + CD20 + B-cell counts were lower in patients with pediatric AD than in control subjects (24% vs 33%, P = .04), whereas CD3 + T-cell counts were higher (62% vs 52%, P = .05). A decreased B-cell/T-cell lymphocyte ratio with age was observed only in pediatric control subjects ( r = −0.48, P = .07). In pediatric patients with AD, a positive correlation was observed between B-cell/T-cell ratio and nonswitched memory B-cell counts ( r = 0.42, P = .03). Higher frequencies of positive sIgE levels were seen in pediatric patients with AD ( P P P H 1, T H 2, total IgE levels, and B-cell memory subsets. Conclusions Peripheral B and T cells are altered in pediatric patients with early AD, but T cells predominate in skin lesions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect