Chicory inulin enhances fermentation of 2′-fucosyllactose by infant fecal microbiota and differentially influences immature dendritic cell and T-cell cytokine responses under normal and Th2-polarizing conditions
| Autor: | Paul de Vos, Erwin G. Zoetendal, Henk A. Schols, Bart J. de Haan, Marijke M. Faas, Madelon J Logtenberg, Martin Beukema, Renate Akkerman |
|---|---|
| Přispěvatelé: | Pharmaceutical Technology and Biopharmacy, Translational Immunology Groningen (TRIGR), Reproductive Origins of Adult Health and Disease (ROAHD), Man, Biomaterials and Microbes (MBM) |
| Rok vydání: | 2021 |
| Předmět: |
T-Lymphocytes
Inulin human feces metagenome Chicory Feces chemistry.chemical_compound Immune system 2'-Fucosyllactose Functional Food Levensmiddelenchemie Humans MolEco Food science VLAG Bifidobacterium chemistry.chemical_classification Food Chemistry biology Chemistry Microbiota Infant Newborn food and beverages General Medicine Dendritic cell Oligosaccharide biology.organism_classification ERP129747 PRJEB45611 Infant Formula Gastrointestinal Microbiome Infant formula Fermentation Trisaccharides Food Science |
| Zdroj: | Food and Function 12 (2021) 19 Food and Function, 12(19), 9018-9029 Food & Function, 12(19). ROYAL SOC CHEMISTRY |
| ISSN: | 2042-650X 2042-6496 |
| DOI: | 10.1039/d1fo00893e |
| Popis: | SCOPE: Non-digestible carbohydrates (NDCs) such as native chicory inulin and 2'-fucosyllactose (2'-FL) are added to infant formula to mimic some of the human milk oligosaccharide (HMO) functions. It is unknown whether combining inulin and 2'-FL influences their fermentation kinetics and whether the immune-modulatory effects of these NDCs are different under normal and inflammatory-prone Th2-polarizing conditions.METHODS AND RESULTS: We investigated the in vitro fermentation of 2'-FL and native chicory inulin, fermented individually and combined, using fecal inocula of 8-week-old infants. Native inulin was fermented in a size-dependent fashion and expedited the fermentation of 2'-FL. Fermentation of both native inulin and 2'FL increased the relative abundance of Bifidobacterium, which coincided with the production of acetate and lactate. The fermentation digesta of all fermentations differentially influenced both dendritic cell and T-cell cytokine responses under normal culture conditions or in presence of the Th2-polarizing cytokines IL-33 and TSLP, with the most pronounced effect for IL-1β in the presence of TSLP.CONCLUSIONS: Our findings show that native inulin can expedite the fermentation of 2'-FL by infant fecal microbiota and that these NDC fermentation digesta have different effects under normal and Th2-polarizing conditions, indicating that infants with different immune backgrounds might benefit from tailored NDC formulations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|