Topography and mobility of ribosomes on the surface of isolated endoplasmic reticulum of rat liver
| Autor: | Gordon Parry, David J. Williams, Carol Davie |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Binding Sites
biology Endoplasmic reticulum Molecular Conformation Endoplasmic Reticulum Ribosome Rats chemistry.chemical_compound Membrane Biochemistry chemistry Liver Structural Biology Polysome biology.protein Biophysics Animals Pancreatic ribonuclease Lamellar structure Glutaraldehyde Binding site Molecular Biology Ribosomes |
| Zdroj: | Journal of molecular biology. 109(4) |
| ISSN: | 0022-2836 |
| Popis: | Ribosome topography was examined on the surface of isolated lamellar rough endoplasmic reticulum. Groups of ribosomes, presumably corresponding to polysomes, were observed as circular, spiral, or parabolic structures. These were distributed fairly randomly on the membrane surface. When samples were incubated at 37°C with pancreatic ribonuclease, conditions that lead to spontaneous aggregation of free ribosomes, clustering of ribosomes to form large aggregates in the plane of the membrane was observed. Prior treatment with glutaraldehyde (5%) prevented this aggregation. It is suggested that ribosome binding components are potentially mobile, but are stabilized in native membranes forming ordered polysome conformations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|