Cardioprotective role of endogenous hydrogen peroxide during ischemia-reperfusion injury in canine coronary microcirculation in vivo
| Autor: | Hidezo Mori, Yoshitaka Morita, Yasuo Ogasawara, Yoshiro Shinozaki, Hiroaki Shimokawa, Toyotaka Yada, Yoshisuke Haruna, Masami Goto, Naoki Kashihara, Fumihiko Kajiya, Osamu Hiramatsu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Nitroprusside Adenosine Nitric Oxide Synthase Type III Physiology Vasodilator Agents Myocardial Infarction Ischemia Vasodilation Pharmacology Nitric Oxide Microcirculation Nitric oxide chemistry.chemical_compound Dogs In vivo Physiology (medical) medicine Animals Myocardial infarction Dose-Response Relationship Drug business.industry Hemodynamics Hydrogen Peroxide medicine.disease Coronary Vessels Acetylcholine chemistry Reperfusion Injury Anesthesia Female Endothelium Vascular Cardiology and Cardiovascular Medicine business Reperfusion injury medicine.drug |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 291:H1138-H1146 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | We have recently demonstrated that endogenous H2O2 plays an important role in coronary autoregulation in vivo. However, the role of H2O2 during coronary ischemia-reperfusion (I/R) injury remains to be examined. In this study, we examined whether endogenous H2O2 also plays a protective role in coronary I/R injury in dogs in vivo. Canine subepicardial small coronary arteries (≥100 μm) and arterioles (G-monomethyl-l-arginine (l-NMMA), catalase (a decomposer of H2O2), 8-sulfophenyltheophylline (8-SPT, an adenosine receptor blocker), l-NMMA + catalase, l-NMMA + tetraethylammonium (TEA, an inhibitor of large-conductance Ca2+-sensitive potassium channels), and l-NMMA + catalase + 8-SPT. Coronary I/R significantly impaired the coronary vasodilatation to ACh in both sized arteries (both P < 0.01); l-NMMA reduced the small arterial vasodilatation (both P < 0.01), whereas it increased ( P < 0.05) the ACh-induced coronary arteriolar vasodilatation associated with fluorescent H2O2 production after I/R. Catalase increased the small arterial vasodilatation ( P < 0.01) associated with fluorescent NO production and increased endothelial NOS expression, whereas it decreased the arteriolar response after I/R ( P < 0.01). l-NMMA + catalase, l-NMMA + TEA, or l-NMMA + catalase + 8-SPT further decreased the coronary vasodilatation in both sized arteries (both, P < 0.01). l-NMMA + catalase, l-NMMA + TEA, and l-NMMA + catalase + 8-SPT significantly increased myocardial infarct area compared with the other four groups (control, l-NMMA, catalase, and 8-SPT; all, P < 0.01). These results indicate that endogenous H2O2, in cooperation with NO, plays an important cardioprotective role in coronary I/R injury in vivo. |
| Databáze: | OpenAIRE |
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