Characterization of antimicrobial peptides in skin secretions from discrete populations of Lithobates chiricahuensis (Ranidae) from central and southern Arizona

Autor: Conlon, J Michael, Mechkarska, Milena, Coquet, Laurent, Jouenne, Thierry, Leprince, Jérôme, Vaudry, Hubert, Kolodziejek, Jolanta, Nowotny, Norbert, King, Jay, Conlon, J. Michael
Přispěvatelé: Faculty of Medicine and Health Science, UAE University, Polymères, biopolymères, membranes (PBM), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), Zoonoses and Emerging Infections Group
Rok vydání: 2011
Předmět:
Erythrocytes
Ranidae
MESH: Sequence Homology
Amino Acid
Physiology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Amino Acid Sequence
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
Biochemistry
0302 clinical medicine
Endocrinology
Anti-Infective Agents
Salientia
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Animals
Skin
Antibacterial agent
MESH: Microbial Sensitivity Tests
0303 health sciences
MESH: Ranidae
biology
MESH: Peptides
Ecology
MESH: Erythrocytes
Arizona
MESH: Hemolysis
Taxonomy (biology)
Mitochondrial DNA
Molecular Sequence Data
MESH: Anti-Infective Agents
Antimicrobial peptides
Zoology
Microbial Sensitivity Tests
Hemolysis
Lithobates chiricahuensis
03 medical and health sciences
Cellular and Molecular Neuroscience
MESH: Skin
Animals
Humans
Amino Acid Sequence
030304 developmental biology
MESH: Humans
MESH: Molecular Sequence Data
Sequence Homology
Amino Acid
Leopard frog
biology.organism_classification
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
Peptides
Frog Skin
030217 neurology & neurosurgery
MESH: Arizona
Zdroj: Peptides
Peptides, Elsevier, 2011, 32 (4), pp.664-669. ⟨10.1016/j.peptides.2011.01.018⟩
ISSN: 0196-9781
DOI: 10.1016/j.peptides.2011.01.018
Popis: International audience; Populations of the Chiricahua leopard frog Lithobates chiricahuensis (Ranidae) occupying regions in southern Arizona (southern range) are morphologically distinct from those from the Mogollon Rim of central Arizona (northern range) and a comparison of DNA sequences of mitochondrial genes has suggested that they may represent separate species. Peptidomic analysis of norepinephrine-stimulated skin secretions has led to the identification of six peptides with antimicrobial activity in samples from specimens from both groups. The primary structure of the peptides (esculentin-2 CHa, ranatuerin-2 CHa, -CHb, and -CHc, and brevinin-1 CHa and -CHb) isolated from both southern and northern range frogs are identical consistent with the proposal that the two populations are conspecific. However, palustrin-2CHa and the atypical brevinin-1 CHc (FFPTIAG*****LTKLFCA ITKKC), containing a five amino acid residue deletion, were identified only in secretions from southern range specimens. Consequently, there is some support for the proposal that the two populations are closely related but separate species but this support is relatively weak. Esculentin-2 CHa (GFSSIFRGVAKFASKGLG KDLAKLGVDLVACKISKQC) displayed the highest antimicrobial potency (MIC ≤ 10μM) against a variety of microorganisms and was only moderately hemolytic (LC(50) = 150 μM). Cladistic analysis based upon the primary structures of brevinin-1 peptides indicates a close phylogenetic relationship between L. chiricahuensis, L. onca, and L. yavapaiensis.
Databáze: OpenAIRE
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