Pancreas Pathology of Latent Autoimmune Diabetes in Adults (LADA) in Patients and in a LADA Rat Model Compared With Type 1 Diabetes
| Autor: | Joachim Jähne, Dirk Wedekind, Sigurd Lenzen, Anne Jörns |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment 030209 endocrinology & metabolism Type 2 diabetes Proinflammatory cytokine 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal Medicine medicine Animals Humans Latent Autoimmune Diabetes in Adults Pancreas Aged Type 1 diabetes business.industry Insulin Interleukin Middle Aged medicine.disease Rats Disease Models Animal Diabetes Mellitus Type 1 030104 developmental biology medicine.anatomical_structure Cytokine Diabetes Mellitus Type 2 Female business |
| Zdroj: | Diabetes. 69:624-633 |
| ISSN: | 1939-327X 0012-1797 |
| Popis: | Approximately 10% of patients with type 2 diabetes suffer from latent autoimmune diabetes in adults (LADA). This study provides a systematic assessment of the pathology of the endocrine pancreas of patients with LADA and for comparison in a first rat model mimicking the characteristics of patients with LADA. Islets in human and rat pancreases were analyzed by immunohistochemistry for immune cell infiltrate composition, by in situ RT-PCR and quantitative real-time PCR of laser microdissected islets for gene expression of proinflammatory cytokines, the proliferation marker proliferating cell nuclear antigen (PCNA), the anti-inflammatory cytokine interleukin (IL) 10, and the apoptosis markers caspase 3 and TUNEL as well as insulin. Human and rat LADA pancreases showed differences in areas of the pancreas with respect to immune cell infiltration and a changed ratio between the number of macrophages and CD8 T cells toward macrophages in the islet infiltrate. Gene expression analyses revealed a changed ratio due to an increase of IL-1β and a decrease of tumor necrosis factor-α. IL-10, PCNA, and insulin expression were increased in the LADA situation, whereas caspase 3 gene expression was reduced. The analyses into the underlying pathology in human as well as rat LADA pancreases provided identical results, allowing the conclusion that LADA is a milder form of autoimmune diabetes in patients of an advanced age. |
| Databáze: | OpenAIRE |
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