Biosynthesis of lens glycoproteins. I. Formation of polyprenyl-linked saccharides in lens cell membranes
| Autor: | Victor Idoyaga-Vargas, Hector Carminatti, Alejandro Mentaberry |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Chromatography
Paper Mannose Chick Embryo Cell membrane Cellular and Molecular Neuroscience chemistry.chemical_compound Column chromatography Biosynthesis Lens Crystalline medicine Animals Nucleotide Asparagine Eye Proteins Glycoproteins Dolichol Phosphates chemistry.chemical_classification Chemistry Cell Membrane Polyisoprenyl Phosphate Oligosaccharides Chromatography Ion Exchange Sensory Systems carbohydrates (lipids) Ophthalmology Paper chromatography Membrane medicine.anatomical_structure Biochemistry Chromatography Thin Layer |
| Zdroj: | Experimental Eye Research. 33:563-576 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/s0014-4835(81)80130-3 |
| Popis: | This work describes the formation of lipid sugar intermediates behaving like dilichyl-P-Glc, dolichyl-P-Man, dolichyl-P-P-GlcNAc and dolichyl-P-P-(GlcNAc) 2 in membranes derived from chick embryonic lens. The reaction products obtained upon incubation of membranes with UDP-[ 14 C]Glc, GDP-[ 14 C]ManandUDP-[ 14 C]GlcNAc were indistinguishables from the corresponding sugar derivatives of rat liver dolichyl-P. This was concluded using the following criteria: (a) effect of detergent, divalent cations, exogenous dolichyl-P and nucleotides on product formation. (b) Lability to acid and alkaline hydrolysis. (c) Behaviour on DEAE-cellulose column chromatography and thin-layer chromatography. This work represents the first step in the approach to elucidate the mechanism of protein glycosylation and the identification of lens glycoproteins of the asparagine type. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|