Copine1 C2 domains have a critical calcium-independent role in the neuronal differentiation of hippocampal progenitor HiB5 cells
| Autor: | Jae Yong Park, Nammi Park, Jae Cheal Yoo, Eun Mi Hwang, Hye Young Choi, Young-Sun Lee, Seong Geun Hong |
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| Rok vydání: | 2014 |
| Předmět: |
Neurite
Biophysics chemistry.chemical_element Biology Calcium Fatty Acid-Binding Proteins Hippocampus Biochemistry Cell Line chemistry.chemical_compound Neural Stem Cells Chlorocebus aethiops Animals Humans Progenitor cell Molecular Biology Protein kinase B Sequence Deletion C2 domain Calcium-Binding Proteins Cell Differentiation Cell Biology Protein subcellular localization prediction Protein Structure Tertiary Rats Cell biology HEK293 Cells chemistry COS Cells Ionomycin Mutant Proteins Signal transduction Carrier Proteins Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 454:228-233 |
| ISSN: | 0006-291X |
| Popis: | Copine1 (CPNE1) has tandem C2 domains and an A domain and is known as a calcium-dependent membrane-binding protein that regulates signal transduction and membrane trafficking. We previously demonstrated that CPNE1 directly induces neuronal differentiation via Akt phosphorylation in the hippocampal progenitor cell line, HiB5. To determine which region of CPNE1 is related to HiB5 cell neurite outgrowth, we constructed several mutants. Our results show that over-expression of each C2 domain of CPNE1 increased neurite outgrowth and expression of the neuronal marker protein neurofilament (NF). Even though protein localization of the calcium binding-deficient mutant of CPNE1 was not affected by ionomycin, this mutant increased neurite outgrowth and NF expression in HiB5 cells. Furthermore, Akt phosphorylation was increased by over-expression of the calcium binding-deficient CPNE1 mutant. These results suggest that neither cellular calcium levels nor the localization of CPNE1 affect its function in neuronal differentiation. Collectively, our findings indicating that the C2 domains of CPNE1 play a calcium-independent role in regulating the neuronal differentiation of HiB5 cells. |
| Databáze: | OpenAIRE |
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