Multi-layered nanofibrous mucoadhesive films for buccal and sublingual administration of drug-delivery and vaccination nanoparticles - important step towards effective mucosal vaccines
| Autor: | František Hubatka, Jana Plocková, Štěpán Koudelka, Eliška Bartheldyová, Andrew D. Miller, Jozsef Gombos, Nongnut Sasithorn, Josef Mašek, Jaroslav Turánek, Milan Raska, Eliška Mašková, Daniela Lubasová, Pavel Kulich, Róbert Lukáč, Pavlina Turanek-Knotigova, Lubomir Prochazka |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Swine Administration Sublingual Nanofibers Pharmaceutical Science Nanoparticle Nanotechnology 02 engineering and technology Polyethylene Glycols Mice 03 medical and health sciences Drug Delivery Systems Adhesives Dendrimer Animals Polyglactin 910 Vaccines Liposome Vaccination Mouth Mucosa Administration Buccal Buccal administration 021001 nanoscience & nanotechnology Electrospinning 030104 developmental biology Pharmaceutical Preparations Liposomes Drug delivery Nanoparticles Lymph Nodes Nanocarriers 0210 nano-technology Layer (electronics) |
| Zdroj: | Journal of Controlled Release. 249:183-195 |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2016.07.036 |
| Popis: | Nanofibre-based mucoadhesive films were invented for oromucosal administration of nanocarriers used for delivery of drugs and vaccines. The mucoadhesive film consists of an electrospun nanofibrous reservoir layer, a mucoadhesive film layer and a protective backing layer. The mucoadhesive layer is responsible for tight adhesion of the whole system to the oral mucosa after application. The electrospun nanofibrous reservoir layer is intended to act as a reservoir for polymeric and lipid-based nanoparticles, liposomes, virosomes, virus-like particles, dendrimers and the like, plus macromolecular drugs, antigens and/or allergens. The extremely large surface area of nanofibrous reservoir layers allows high levels of nanoparticle loading. Nanoparticles can either be reversibly adsorbed to the surface of nanofibres or they can be deposited in the pores between the nanofibres. After mucosal application, nanofibrous reservoir layers are intended to promote prolonged release of nanoparticles into the submucosal tissue. Reversible adsorption of model nanoparticles as well as sufficient mucoadhesive properties were demonstrated. This novel system appears appropriate for the use in oral mucosa, especially for sublingual and buccal tissues. To prove this concept, trans-/intramucosal and lymph-node delivery of PLGA-PEG nanoparticles was demonstrated in a porcine model. This system can mainly be used for sublingual immunization and the development of "printed vaccine technology". |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect