Constellation: a tool for rapid, automated phenotype assignment of a highly polymorphic pharmacogene, CYP2D6, from whole-genome sequences
| Autor: | Julie A. Cakici, Darrell L. Dinwiddie, Laurel K. Willig, Margaret Gibson, J. Steven Leeder, Josh E Petrikin, Stephen F. Kingsmore, Amanda K. Riffel, Andrea Gaedigk, Suzanne Herd, Greyson P Twist, Emily G. Farrow, Deendayal Dinakarpandian, Sarah E Soden, Neil A. Miller |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Sanger sequencing Genetics 0303 health sciences Haplotype Locus (genetics) Biology Genome Article 3. Good health 03 medical and health sciences symbols.namesake 0302 clinical medicine 030104 developmental biology 030220 oncology & carcinogenesis Genetic variation Gene duplication symbols Corrigendum Molecular Biology Gene Genotyping Genetics (clinical) 030304 developmental biology |
| Zdroj: | NPJ Genomic Medicine |
| ISSN: | 2056-7944 |
| DOI: | 10.1038/npjgenmed.2015.7 |
| Popis: | An important component of precision medicine—the use of whole-genome sequencing (WGS) to guide lifelong healthcare—is electronic decision support to inform drug choice and dosing. To achieve this, automated identification of genetic variation in genes involved in drug absorption, distribution, metabolism, excretion and response (ADMER) is required. CYP2D6 is a major enzyme for drug bioactivation and elimination. CYP2D6 activity is predominantly governed by genetic variation; however, it is technically arduous to haplotype. Not only is the nucleotide sequence of CYP2D6 highly polymorphic, but the locus also features diverse structural variations, including gene deletion, duplication, multiplication events and rearrangements with the nonfunctional, neighbouring CYP2D7 and CYP2D8 genes. We developed Constellation, a probabilistic scoring system, enabling automated ascertainment of CYP2D6 activity scores from 2×100 paired-end WGS. The consensus reference method included TaqMan genotyping assays, quantitative copy-number variation determination and Sanger sequencing. When compared with the consensus reference Constellation had an analytic sensitivity of 97% (59 of 61 diplotypes) and analytic specificity of 95% (116 of 122 haplotypes). All extreme phenotypes, i.e., poor and ultrarapid metabolisers were accurately identified by Constellation. Constellation is anticipated to be extensible to functional variation in all ADMER genes, and to be performed at marginal incremental financial and computational costs in the setting of diagnostic WGS. |
| Databáze: | OpenAIRE |
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