Structure of a kallikrein-like enzyme and its tissue localization in the dog
| Autor: | Akio Kinoshita, Kikuo Arakawa, Keita Noda, Emiko Tsuji, Shigenori Ogata, Munehito Ideishi, Manabu Sasaguri, Manabu Koga |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Glycosylation Blotting Western Aorta Thoracic Biology Kidney Peptide Mapping Dogs Sequence Analysis Protein Internal medicine Renin–angiotensin system medicine Animals Amino Acids Pharmacology chemistry.chemical_classification urogenital system Myocardium Angiotensin-converting enzyme Kallikrein Kinin Molecular biology Small intestine Enzyme Activation Molecular Weight Blot Enzyme medicine.anatomical_structure Endocrinology chemistry Organ Specificity biology.protein Kallikreins circulatory and respiratory physiology |
| Zdroj: | Immunopharmacology. 44:15-19 |
| ISSN: | 0162-3109 |
| Popis: | We previously purified a kallikrein-like enzyme from the dog heart and demonstrated that it is not only able to form kinins but can also convert angiotensin (Ang) I to Ang II. The aim of the present study was to clarify the structure and tissue localization of this enzyme. Western blot analysis of various canine tissues was performed with antiserum against the purified dog heart enzyme. The purified enzyme was subjected to a determination of its amino acid composition and a sequence analysis. Western blotting indicated that this enzyme was present in the heart, aorta, kidney, pancreas, lung, liver, spleen, small intestine, and skeletal muscle. The amino acid composition of the enzyme was different from that of dog urinary kallikrein. Amino acid sequence analysis indicated that it is likely to be N-terminally blocked. The present study showed that this kallikrein-like enzyme is different from previously reported kallikrein and is distributed not only in the heart, but also in other tissues such as the aorta, kidney, lung, liver, spleen, small intestine, and skeletal muscle. This enzyme may exert local effects by generating kinins and Ang II. |
| Databáze: | OpenAIRE |
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