Expression of follicle-stimulating hormone and luteinising hormone binding sites in the bitch ovary during the follicular phase
| Autor: | Marie Saint-Dizier, Sandra Thoumire, Nina Jaffré, Sylvie Chastant-Maillard, Benoit Remy, Karine Reynaud |
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| Přispěvatelé: | Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), AgroParisTech, Université de Liège, École nationale vétérinaire d'Alfort (ENVA) |
| Rok vydání: | 2008 |
| Předmět: |
LH
endocrine system medicine.medical_specialty media_common.quotation_subject FOLLICULE OVARIEFSH Ovary Biology Oogenesis 03 medical and health sciences Follicle-stimulating hormone Follicle Dogs 0302 clinical medicine Endocrinology Growing Follicle Internal medicine Follicular phase Genetics medicine Animals HORMONE GONADOTROPE FOLLICLE STIMULATING HORMONE Molecular Biology Ovulation 030304 developmental biology media_common 0303 health sciences Granulosa Cells 030219 obstetrics & reproductive medicine FEMALE DOGS [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Luteinizing Hormone Receptors LH medicine.anatomical_structure Follicular Phase Reproductive Medicine Theca Cells OVARY Receptors FSH Female Animal Science and Zoology OVULATION Folliculogenesis Protein Binding Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development Reproduction, Fertility and Development, CSIRO Publishing, 2008, 20, pp.925-934. ⟨10.1071/RD08119⟩ |
| ISSN: | 1031-3613 |
| Popis: | International audience; In the female dog, in contrast with most mammals, the growing follicle starts to luteinise several days before ovulation. Little is known about the physiological control of the final follicular growth in this species. In order to better understand the pituitary regulation of follicular growth, specific binding sites for FSH and LH were localised and quantified by autoradiography using [(125)I]-porcine (p) gonadotrophins on ovarian sections (7 microm) from adult Beagle bitches during the follicular phase. Follicles were analysed either before the LH surge (n = 4 bitches; n = 117 follicles) or after the LH surge and before ovulation (n = 5 bitches; n = 110 follicles). FSH binding sites were specifically and homogeneously expressed at high levels on granulosa cells of all healthy follicles from the preantral stage onwards. In contrast, LH binding sites were detected homogeneously and at high levels only on granulosa cells of follicles larger than 1 mm in diameter, including luteinised follicles. Theca binding of LH (but not FSH) was also observed, but only when using high concentrations of [(125)I]-pLH. The overall incidence of atresia was 45.8% and was dependent upon follicular diameter. Quantitative analysis of labelling showed that atretic follicles had reduced levels of both FSH and LH binding sites compared with healthy follicles. In healthy follicles, levels of both FSH and LH binding sites changed with follicle diameter. Compared with other mammals, the acquisition of LH binding on canine granulosa cells occurs in smaller sized follicles relative to the size of ovulation. |
| Databáze: | OpenAIRE |
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