Sendai virus induced cytoplasmic actin remodeling correlates with efficient virus particle production
| Autor: | Laurent Roux, Geneviève Mottet-Osman, Vincent Miazza, Christine Chaponnier, Sergei Startchick |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Gene Expression Regulation
Viral Cytoplasm M protein viruses Virus Replication/physiology Virus morphogenesis macromolecular substances ddc:616.07 Virus Replication Virion/physiology Sendai virus Virus ESCRT Actin remodeling Cell Line Viral Matrix Proteins 03 medical and health sciences Paramyxovirus Dogs Viral envelope Virology Gene Expression Regulation Viral/physiology Sendai virus/physiology Animals Protein Isoforms RNA Small Interfering Actin 030304 developmental biology ddc:616 0303 health sciences Viral matrix protein Microscopy Confocal biology 030306 microbiology Virion biology.organism_classification Actins Cell biology Cytoplasm/metabolism Polarized cells Viral replication Actins/metabolism Viral Matrix Proteins/genetics/metabolism |
| Zdroj: | Virology Virology, Vol. 410, No 1 (2011) pp. 7-16 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2010.10.003 |
| Popis: | Cytoplasmic actins have been found interacting with viral proteins and identified in virus particles. We analyzed by confocal microscopy the cytoplasmic β- and γ-actin patterns during the course of Sendai virus infections in polarized cells. We observed a spectacular remodeling of the β-cytoplasmic actin which correlated with productive viral multiplication. Conversely, suppression of M during the course of a productive infection resulted in the decrease of particle production and the absence of β-actin remodeling. As concomitant suppression of β- and γ-actins resulted as well in reduction of virus particle production, we propose that Sendai virus specifically induces actin remodeling in order to promote efficient virion production. Beta- and γ-cytoplasmic actin recruitment could substitute for that of the endosomal sorting complex required for transport (ESCRT) mobilized by other enveloped viruses but apparently not used by Sendai virus. |
| Databáze: | OpenAIRE |
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