Possibility of Japanese Cedar Pollen Causing False Positives in the Deep Mycosis Test
| Autor: | Ken-ichi Ishibashi, Yoshiyuki Adachi, Takashi Kanno, Naohito Ohno, Hiroshi Tanaka, Daisuke Yamanaka, Changmin Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
beta-Glucans Cryptomeria 030106 microbiology Catalysis Article Inorganic Chemistry lcsh:Chemistry 03 medical and health sciences Japanese cedar pollen False positive paradox False Positive Reactions Lectins C-Type Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy Chromatography Chemistry Organic Chemistry General Medicine Hydrogen-Ion Concentration Deep mycosis beta-d-glucan Computer Science Applications Limulus amebocyte lysate assay glucan-binding protein 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Mycoses Limulus amebocyte lysate pollen Beta d glucan |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 4 International Journal of Molecular Sciences, Vol 22, Iss 2135, p 2135 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Because Japanese cedar pollen (JCP) contains beta-1,3-d-glucan (BG), there is concern that its lingering presence in the atmosphere, especially during its scattering period, may cause false positives in the factor-G-based Limulus amebocyte lysate (LAL) assay used to test for deep mycosis (i.e., G-test). Hence, we examined whether the LAL assay would react positively with substances contained in JCP by using the G-test to measure JCP particles and extracts. BG was purified from the JCP extract on a BG-specific affinity column, and the percentage extractability was measured using three different BG-specific quantitative methods. The G-test detected 0.4 pg BG in a single JCP particle and 10 fg from a single particle in the extract. The percentage extractability of JCP-derived BG was not significantly different among the three quantitative methods. As the JCP particles should technically have been removed during serum separation, they should be less likely to be a direct false-positive factor. However, given that the LAL-assay-positive substances in the JCP extract were not distinguishable by the three BG-specific quantitative methods, we conclude that they may cause the background to rise. Therefore, in Japan false positives arising from JCP contamination should be considered when testing patients for deep mycosis. |
| Databáze: | OpenAIRE |
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