Do G-protein coupled estrogen receptor and bisphenol A analogs influence on Leydig cell epigenetic regulation in immature boar testis ex vivo?
| Autor: | Malgorzata Kotula-Balak, Barbara Bilińska, Michal Duliban, Waclaw Tworzydlo, R. Tuz, Ewelina Gorowska-Wojtowicz, Anna Ptak, Agnieszka Milon, Katarzyna Knapczyk-Stwora, Bartosz J. Płachno, P. Pawlicki |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Swine bisphenol A boar Estrogen receptor Epigenesis Genetic Receptors G-Protein-Coupled 03 medical and health sciences MicroRNA biogenesis and function controlling molecules 0302 clinical medicine Endocrinology Leydig cell Food Animals Downregulation and upregulation Phenols Internal medicine Lipid droplet Testis medicine Animals Sexual Maturation Benzhydryl Compounds Drosha 030219 obstetrics & reproductive medicine biology Chemistry 0402 animal and dairy science Gene Expression Regulation Developmental Leydig Cells 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Estradiol secretion MicroRNAs medicine.anatomical_structure Receptors Estrogen biology.protein Animal Science and Zoology Gene-Environment Interaction G-coupled membrane estrogen receptor GPER hormones hormone substitutes and hormone antagonists Dicer |
| Popis: | Organotypic culture of testicular fragments from 7-day-old male pigs (Polish White Large) was used. Tissues were treated with an antagonist of G-protein coupled estrogen receptor (GPER) (G-15; 10 nM), and bisphenol A (BPA), and its analogs (TBBPA, TCBPA; 10 nM) alone or in combination and analyzed using electron and light (stainings for collagen fibers, lipid droplet and autophagy markers) microscopes. In addition, mRNA and protein abundances and localization of molecules required for miRNA biogenesis and function (Drosha, Exportin 5; EXPO5, Dicer, and Argonaute 2; AGO2) were assessed together with calcium ion (Ca2+) and estradiol concentrations. Regardless of GPER blockade and/or treatment with BPA, TBBPA and TCBPA, there were no changes in Leydig cell morphology. Also, there were no changes in lipid droplet content and distribution but there were changes in lipid and autophagy protein abundance. In the interstitial tissue, there was an increase of collagen content, especially after treatment with BPA analogs and G-15 + BPA. Independent of the treatment, there was downregulation of EXPO5 and Dicer genes but the Drosha and AGO2 genes were markedly upregulated as a result of treatment with G-15 + BPA and TCBPA, respectively. There was always a lesser abundance of EXPO5 and AGO2 proteins regardless of treatment. There was markedly greater abundances of Drosha after G-15 + BPA treatment, and this also occurred for Dicer after treatment with G-15 + TCBPA. Immunolocalization of miRNA proteins indicated there was a cytoplasmic-nuclear pattern in control and treated cells. There was an increase of Ca2+ concentrations after treatment with G-15 and BPA analogs. Estradiol secretion decreased after antagonist and chemical treatments when these were administered alone, however, there was an increase in estradiol secretion after treatment with combinations of these compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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