| Autor: |
A. Kubicz, Irena Lorenc-Kubis, Bronisława Morawiecka, E. Wieczorek |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Acta Societatis Botanicorum Poloniae, Vol 45, Iss 1–2, Pp 111-117 (2015) |
| ISSN: |
2083-9480 |
| DOI: |
10.5586/asbp.1976.010 |
| Popis: |
The contents of sugars. proteins and acid phosphatase extracted with 0.1 M acetate buffer, pH 5.1, from some rye varieties were determined. The total sugar level amounted to 3.25-9.70 g per 100 g of seeds; the estimates for pentoses were 1.7-2.9 g and those for proteins 0.91-1.60 g per 100 g of seeds. Acid phosphatase showed and activity level between 0.18 and 1.26 units/mg protein. After disc electrophoresis proteins were separated into 10 to 11 bands: at pH 9.4 or into 4-7 bands at pH 3.8. Essential variety differences were expressed in protein patterns after electrophoresis at pH 3.8. Acid phosphatase was separated into 5 and 4 activity bands at pH 9.4 and 3.3,. respectively. No variations in zymogram patterns were observed in respect to variety differences or cultivation in various climate and soil conditions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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