Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China
| Autor: | Shuqing Zuo, Xucan Zeng, Yigang Tong, Jiusong Zhang, Honghong Peng, Xiaofang Guo, Ke Dai, Yuanyuan Wang, Yunlan Lu, Hongning Zhou |
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| Rok vydání: | 2018 |
| Předmět: |
China
Culex Insect Viruses Genome Viral Genome Virus 03 medical and health sciences Viral Proteins Virus strain Phylogenetics Virology Animals RNA Viruses Phylogeny 030304 developmental biology Whole genome sequencing Genetics 0303 health sciences biology Phylogenetic tree Base Sequence Whole Genome Sequencing 030306 microbiology General Medicine biology.organism_classification Culex tritaeniorhynchus |
| Zdroj: | Archives of virology. 164(3) |
| ISSN: | 1432-8798 |
| Popis: | A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus. |
| Databáze: | OpenAIRE |
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