Interaction of Mycoplasma hominis PG21 with human dendritic cells: IL-23-inducing mycoplasmal lipoproteins and inflammasome activation of the cell
| Autor: | C. Le Roy, Laure Béven, H. Renaudin, Benjamin Faustin, Cécile Contin-Bordes, Sabine Pereyre, Cécile Bébéar, Stéphane Claverol, Julien Goret |
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| Přispěvatelé: | USC EA 3671 Mycoplasmal and chlamydial infections in humans, Université de Bordeaux (UB), Institut National de la Recherche Agronomique (INRA), Laboratoire de Bactériologie, Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, CNRS UMR 5164, Centre de Génomique Fonctionnelle de Bordeaux (CGFB), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Inflammasomes Cell Chemical Fractionation Interleukin-23 Polymerase Chain Reaction Mass Spectrometry IL-23 Cells Cultured Inflammasome Mycoplasma hominis medicine.anatomical_structure IL-1β Host-Pathogen Interactions Electrophoresis Polyacrylamide Gel bactérie pathogène Research Article medicine.drug caspase Detergents 030106 microbiology interaction Enzyme-Linked Immunosorbent Assay Biology Microbiology 03 medical and health sciences Immune system Bacterial Proteins inflammasome medicine Extracellular Humans Secretion mollicute Molecular Biology Innate immune system PCR array pathogenic bacteria Dendritic Cells Microarray Analysis biology.organism_classification Immunity Innate Molecular Weight lipoproteins 030104 developmental biology lipopeptide innate response human dendritic cells [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Lipoprotein |
| Zdroj: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2017, 199 (15), pp.e00213-17. ⟨10.1128/JB.00213-17⟩ |
| ISSN: | 0021-9193 1098-5530 |
| Popis: | Mycoplasma hominis lacks a cell wall, and lipoproteins anchored to the extracellular side of the plasma membrane are in direct contact with the host components. A Triton X-114 extract of M. hominis enriched with lipoproteins was shown to stimulate the production of interleukin-23 (IL-23) by human dendritic cells (hDCs). The inflammasome activation of the host cell has never been reported upon M. hominis infection. We studied here the interaction between M. hominis PG21 and hDCs by analyzing both the inflammation-inducing mycoplasmal lipoproteins and the inflammasome activation of the host cell. IL-23-inducing lipoproteins were determined using a sequential extraction strategy with two nondenaturing detergents, Sarkosyl and Triton X-114, followed by SDS-PAGE separation and mass spectrometry identification. The activation of the hDC inflammasome was assessed using PCR array and enzyme-linked immunosorbent assay (ELISA). We defined a list of 24 lipoproteins that could induce the secretion of IL-23 by hDCs, 5 with a molecular mass between 20 and 35 kDa and 19 with a molecular mass between 40 and 100 kDa. Among them, lipoprotein MHO_4720 was identified as potentially bioactive, and a synthetic lipopeptide corresponding to the N-terminal part of the lipoprotein was subsequently shown to induce IL-23 release by hDCs. Regarding the hDC innate immune response, inflammasome activation with caspase-dependent production of IL-1β was observed. After 24 h of coincubation of hDCs with M. homini s, downregulation of the NLRP3-encoding gene and of the adaptor PYCARD-encoding gene was noticed. Overall, this study provides insight into both protagonists of the interaction of M. hominis and hDCs. IMPORTANCE Mycoplasma hominis is a human urogenital pathogen involved in gynecologic and opportunistic infections. M. hominis lacks a cell wall, and its membrane contains many lipoproteins that are anchored to the extracellular side of the plasma membrane. In the present study, we focused on the interaction between M. hominis and human dendritic cells and examined both sides of the interaction, the mycoplasmal lipoproteins involved in the activation of the host cell and the immune response of the cell. On the mycoplasmal side, we showed for the first time that M. hominis lipoproteins with high molecular mass were potentially bioactive. On the cell side, we reported an activation of the inflammasome, which is involved in the innate immune response. |
| Databáze: | OpenAIRE |
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