Rosmarinic Acid from Eelgrass Shows Nematicidal and Antibacterial Activities against Pine Wood Nematode and Its Carrying Bacteria
| Autor: | Jingyu Wang, Daosen Guo, Qunqun Guo, Xueru Pan, Yi Han, Ronggui Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
rosmarinic acid
Magnetic Resonance Spectroscopy Time Factors Nematoda Pharmaceutical Science Bursaphelenchus xylophilus Depsides Article chemistry.chemical_compound anti-bacterial activity Drug Discovery Botany Animals Zostera Nematode Infections Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 Chromatography High Pressure Liquid Wilt disease Plant Diseases biology Plant Extracts Rosmarinic acid Antinematodal Agents Zosteraceae Extraction (chemistry) Zostera marina biology.organism_classification Pinus nematicidal activity Pantoea agglomerans Anti-Bacterial Agents Horticulture chemistry lcsh:Biology (General) Cinnamates Crystallization Bacteria |
| Zdroj: | Marine Drugs, Vol 10, Iss 12, Pp 2729-2740 (2012) Marine Drugs Marine Drugs; Volume 10; Issue 12; Pages: 2729-2740 |
| ISSN: | 1660-3397 |
| Popis: | Pine wilt disease (PWD), a destructive disease for pine trees, is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus and additional bacteria. In this study, extracts of Zostera marina showed a high nematicidal activity against PWN and some of the bacteria that it carries. Light yellow crystals were obtained from extracts of Z. marina through solvent extraction, followed by chromatography on AB-8 resin and crystallization. The NMR and HPLC analysis showed that the isolated compound was rosmarinic acid (RosA). RosA showed effective nematicidal activity, of which the LC50 (50% lethal concentration) to PWN at 24 h, 48 h and 72 h was 1.18 mg/g, 1.05 mg/g and 0.95 mg/g, respectively. To get a high yield rate of RosA from Z. marina, single factor experiments and an L9 (34) orthogonal experiment were performed. This extraction process involved 70% ethanol for 3 h at 40 °C. The extraction dosage was 1:50 (w/v). The highest yield of RosA from Zostera was 3.13 mg/g DW (dried weight). The crude extracts of Zostera marina (10 mg/mL) and RosA (1 mg/mL) also showed inhibitory effects to some bacterial strains carried by PWN: Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp. and Pantoea agglomerans. The results of these studies provide clues for preparing pesticide to control PWD from Z. marina. |
| Databáze: | OpenAIRE |
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